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Human Naïve Pluripotent Stem Cells

  • Book
  • © 2022


  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2416)

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Table of contents (17 protocols)


About this book

This volume provides readers with a comprehensive collection of methods to guide them on how to generate, characterize, and use naïve human pluripotent stem cells (hPSCs). The chapters in this book cover topics such as three predominant routes to generate naïve hPSC lines; methods to differentiate naïve hPSCs into specialized cell types; and techniques to characterize naïve hPSCs using key molecular landmarks that benchmark and quality control the cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and thorough, Human Naïve Pluripotent Stem Cells: Methods and Protocols is a valuable resource for novice and expert researchers who are looking to learn more or expand their research in this developing field.

Editors and Affiliations

  • Babraham Institute, Cambridge, UK

    Peter Rugg-Gunn

Bibliographic Information

  • Book Title: Human Naïve Pluripotent Stem Cells

  • Editors: Peter Rugg-Gunn

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1907-0Published: 07 December 2021

  • Softcover ISBN: 978-1-0716-1910-0Published: 08 December 2022

  • eBook ISBN: 978-1-0716-1908-7Published: 06 December 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 288

  • Number of Illustrations: 66 b/w illustrations

  • Topics: Stem Cells, Regenerative Medicine/Tissue Engineering, Cell Biology

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