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Essential Genes and Genomes

Methods and Protocols

Editors:

  • Includes cutting-edge techniques

  • Provides step-by-step detail essential for reproducible results

  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2377)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xii
  2. A Method to Map Gene Essentiality of Human Pluripotent Stem Cells by Genome-Scale CRISPR Screens with Inducible Cas9

    • Barbara Mair, Michael Aregger, Amy H. Y. Tong, Katherine S. K. Chan, Jason Moffat
    Pages 1-27
  3. CRISPR/Cas9 Screening to Identify Conditionally Essential Genes in Human Cell Lines

    • Kimberly S. Huggler, Nicholas J. Rossiter, Kyle M. Flickinger, Jason R. Cantor
    Pages 29-42
  4. Identification of Essential Genes Using Sequential CRISPR and siRNA Screens

    • Luke DeHart, Oliver P. Yockey, Jesse Bakke
    Pages 89-107
  5. A CRISPR-Based Method for Constructing Conditional Mutations of Essential Genes in Cyanobacteria

    • Ju-Yuan Zhang, Tian-Cai Niu, Gui-Ming Lin, Cheng-Cai Zhang
    Pages 143-157
  6. The Use of Tn-Seq and the FiTnEss Analysis to Define the Core Essential Genome of Pseudomonas aeruginosa

    • Bradley E. Poulsen, Anne E. Clatworthy, Deborah T. Hung
    Pages 179-197
  7. Using Genome Scale Mutant Libraries to Identify Essential Genes

    • Kevin S. Myers, Piyush Behari Lal, Daniel R. Noguera, Timothy J. Donohue
    Pages 215-236
  8. Repressible Promoter System to Study Essential Genes in Mycobacteria

    • Francesca Boldrin, Riccardo Manganelli
    Pages 317-332

About this book

This book provides state-of-the-art information on gene essentiality screenings in a wide variety of organisms, i.e. screening for protein-coding genes and other genomic elements that are required by an organism to survive under specific conditions. With a focus on the two techniques that have revolutionized the field, the collection begins with chapters employing CRISPR/Cas9-based approaches followed by Tn-seq-based approaches, but later chapters also delve into other techniques for exploring essential genes, such as bioinformatics methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Comprehensive and authoritative, Essential Genes and Genomes: Methods and Protocols is an ideal guide for researchers attempting to strip genetics down to its fundamentals.

Keywords

  • CRISPR/Cas9
  • Genome-wide essentiality screening
  • Gene essentiality
  • Synthetic biology
  • Gene targeting technology
  • Database of essential genes (DEG)

Editors and Affiliations

  • Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, USA

    Ren Zhang

Bibliographic Information

  • Book Title: Essential Genes and Genomes

  • Book Subtitle: Methods and Protocols

  • Editors: Ren Zhang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1720-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1719-9Published: 29 October 2021

  • Softcover ISBN: 978-1-0716-1722-9Published: 29 October 2022

  • eBook ISBN: 978-1-0716-1720-5Published: 28 October 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 434

  • Number of Illustrations: 18 b/w illustrations, 50 illustrations in colour

  • Topics: Genetics and Genomics, Chemistry/Food Science, general, Chemistry/Food Science, general

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access