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Parasite Genomics

Methods and Protocols

  • Includes cutting-edge techniques

  • Provides step-by-step detail essential for reproducible results

  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2369)

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  • ISBN: 978-1-0716-1681-9
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Softcover Book USD 159.99
Price excludes VAT (USA)
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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
  2. Novel Sequencing and Bioinformatic Pipelines for the Study of Parasite Genomes

    1. Front Matter

      Pages 1-1
    2. Nanopore Long Read DNA Sequencing of Protozoan Parasites: Hybrid Genome Assembly of Trypanosoma cruzi

      • Florencia Díaz-Viraqué, Gonzalo Greif, Luisa Berná, Carlos Robello
      Pages 3-13
    3. Automated Phylogenetic Analysis Using Best Reciprocal BLAST

      • Erin R. Butterfield, James C. Abbott, Mark C. Field
      Pages 41-63
  3. Diagnostic Approaches Using Genomic Tools

    1. Front Matter

      Pages 65-65
    2. Giardia duodenalis: Detection by Quantitative Real-Time PCR and Molecular Diversity

      • Alejandro Dashti, Pamela C. Köster, David Carmena
      Pages 83-97
  4. Host-Parasite Interactions: Deciphering Gene Function and Molecular Processes in Parasites

    1. Front Matter

      Pages 99-99
    2. Conditional Gene Deletion in Mammalian and Mosquito Stages of Plasmodium berghei Using Dimerizable Cre Recombinase

      • Priyanka Fernandes, Manon Loubens, Olivier Silvie, Sylvie Briquet
      Pages 101-120
    3. Genome-Wide Analysis of RNA–Protein Interactions in Plasmodium falciparum Using eCLIP-Seq

      • Thomas Hollin, Steven Abel, Karine G. Le Roch
      Pages 139-164
    4. Transcriptional Analysis of Tightly Synchronized Plasmodium falciparum Intraerythrocytic Stages by RT-qPCR

      • Núria Casas-Vila, Anastasia K. Pickford, Harvie P. Portugaliza, Elisabet Tintó-Font, Alfred Cortés
      Pages 165-185
    5. Integration of Genomic and Transcriptomic Data to Elucidate Molecular Processes in Babesia divergens

      • Luis Miguel Gonzalez, Elena Sevilla, Miguel Fernández-García, Alejandro Sanchez-Flores, Estrella Montero
      Pages 199-215
    6. Integration of Functional Genomic, Transcriptomic, and Metabolomic Data to Identify Key Features in Genomic Expression, Metabolites, and Metabolic Pathways of Babesia divergens

      • Miguel Fernández-Garcia, Alejandro Sanchez-Flores, Luis Miguel Gonzalez, Coral Barbas, Mª. Fernanda Rey-Stolle, Elena Sevilla et al.
      Pages 217-249
    7. Helminth Microbiota Profiling Using Bacterial 16S rRNA Gene Amplicon Sequencing: From Sampling to Sequence Data Mining

      • Fabio Formenti, Gabriel Rinaldi, Cinzia Cantacessi, Alba Cortés
      Pages 263-298
  5. Genomics of Parasite-Derived Extracellular Vesicles

    1. Front Matter

      Pages 299-299

About this book

This detailed book provides a comprehensive series of innovative research techniques and methodologies applied to the parasite genomics research area, all applying different approaches to analyzing parasite genomes and furthering the study of genetic complexity and the mechanisms of regulation. Beginning with chapters on novel sequencing and the bioinformatics pipeline, the volume continues by exploring diagnostic approaches using genomic tools, host-parasite interactions, as well as the genomics of parasite-derived extracellular vesicles. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Parasite Genomics: Methods and Protocols creates a detailed picture of genomic approaches for researchers seeking a better understanding of characterizing parasite nucleic acid content.

Keywords

  • Sequencing
  • Diagnostic approaches
  • Host-parasite interactions
  • Parasite-derived extracellular vesicles
  • Gene function
  • Regulatory networks
  • Genetic complexity

Editors and Affiliations

  • Departamento de Parasitologia, Universidad de Granada, Granada, Spain

    Luis M. de Pablos

  • Laboratorio de Referencia e Investigación en Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Spain

    Javier Sotillo

Bibliographic Information

  • Book Title: Parasite Genomics

  • Book Subtitle: Methods and Protocols

  • Editors: Luis M. de Pablos, Javier Sotillo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1681-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1680-2

  • Softcover ISBN: 978-1-0716-1683-3

  • eBook ISBN: 978-1-0716-1681-9

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 336

  • Number of Illustrations: 12 b/w illustrations, 25 illustrations in colour

  • Topics: Parasitology, Microbial Genetics, Genetics and Genomics

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • ISBN: 978-1-0716-1681-9
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 159.99
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)