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Imaging and Quantifying Neuronal Autophagy

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  • © 2022

Overview

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 171)

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Table of contents (10 protocols)

Keywords

About this book

The volume aims to explore the dynamic nature of the autophagy pathway, and the latest techniques that allow researchers to capture and quantify this process in neurons. The chapters in this volume cover topics such as fundamental, historical, and functional approaches that began in baker’s yeast; Saccharomyces cerevisiae; the role of both electron microscopy and live-cell imaging using fluorescently tagged autophagy proteins; and the rate of puncta appearance and its correlation with the rate of autophagosome formation. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. 

Cutting-edge and practical, Imaging and Quantifying Neuronal is a valuable resource that provides insights into the power of microscopy tools, live-cell imaging, and photoactivation and correlative techniques. 


Editors and Affiliations

  • Department of Physiological Sciences, Stellenbosch University, Stellenbosch, South Africa

    Ben Loos

  • Healthy Longevity Translational Research Programme, Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Centre for Healthy Longevity National University Health System, Singapore, Singapore

    Esther Wong

Bibliographic Information

  • Book Title: Imaging and Quantifying Neuronal Autophagy

  • Editors: Ben Loos, Esther Wong

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-0716-1589-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-1588-1Published: 20 August 2021

  • Softcover ISBN: 978-1-0716-1591-1Published: 21 August 2022

  • eBook ISBN: 978-1-0716-1589-8Published: 19 August 2021

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XII, 150

  • Number of Illustrations: 6 b/w illustrations, 27 illustrations in colour

  • Topics: Neurosciences

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