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Fibrous Proteins

Design, Synthesis, and Assembly

  • Book
  • © 2021

Overview

Editors:
  1. Shengjie Ling

    1. School of Physical Science and Technology, ShanghaiTech University, Shanghai, China

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2347)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-x
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  2. Introduction to Fibrous Proteins

    1. Front Matter

      Pages 1-1
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    2. Structure of Animal Silks

      • Wenwen Zhang, Yimin Fan
      Pages 3-15

    3. Structure of Collagen

      • Chenxi Zhao, Yuelong Xiao, Shengjie Ling, Ying Pei, Jing Ren
      Pages 17-25

    4. Structure of Elastin

      • Yuelong Xiao, Shengjie Ling, Ying Pei
      Pages 27-33

    5. Structure of Resilin

      • Yuelong Xiao, Shengjie Ling, Ying Pei
      Pages 35-40

    6. Structure of Keratin

      • Wenwen Zhang, Yimin Fan
      Pages 41-53

  3. Methods in Synthesis of Fibrous Proteins

    1. Front Matter

      Pages 55-55
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    2. General Methods to Produce and Assemble Recombinant Spider Silk Proteins

      • Na Kong
      Pages 57-67

    3. Self-Assembly of Bombyx mori Silk Fibroin

      • Na Kong
      Pages 69-82

    4. Synthesis and Assembly of Recombinant Collagen

      • Chenxi Zhao, Yuelong Xiao, Shengjie Ling, Ying Pei, Jing Ren
      Pages 83-96

    5. Recombination and Purification of Elastin-Like Polypeptides

      • Chenxi Zhao, Yuelong Xiao, Shengjie Ling, Ying Pei, Jing Ren
      Pages 97-103

    6. Methods to Synthesize and Assemble Recombinant Keratins

      • Wenwen Zhang, Yimin Fan
      Pages 105-112

    7. Preparation of Amyloid Fibrils Using Recombinant Technology

      • Wenwen Zhang, Yimin Fan
      Pages 113-121

    8. Molecular Biology Methods to Construct Recombinant Fibrous Protein

      • Jicong Zhang
      Pages 123-135

  4. Methods in Assembly of Fibrous Proteins

    1. Front Matter

      Pages 137-137
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    2. Isolation of Nanofibrils from Animal Silks

      • Ke Zheng
      Pages 139-148

    3. Gelation Methods to Assemble Fibrous Proteins

      • Ning Fan, Ke Zheng
      Pages 149-165

    4. Spinning Methods Used for Construction of One- and Two-Dimensional Fibrous Protein Materials

      • Leitao Cao
      Pages 167-176

    5. Three-Dimensional Printing to Build Fibrous Protein Architectures

      • Huanhuan Qiao, Ke Zheng
      Pages 177-189
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Keywords

About this book

This detailed volume explores fibrous proteins widely present in different biological tissues or biological structural materials. The book begins by introducing the structure of representative fibrous proteins, including animal silks, collagen, elastin, resilin, and keratin, and it then continues by providing detailed experimental protocols for the synthesis, assembly, and characterization of natural, regenerated, and recombinant fibrous proteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Fibrous Proteins: Design, Synthesis, and Assembly is an ideal guide for researchers aiming to master fibrous protein preparations with the aid of this broad and interdisciplinary perspective on understanding the structure-property-function relationships of natural and reconstituted fibrous proteins.

Editors and Affiliations

  • School of Physical Science and Technology, ShanghaiTech University, Shanghai, China

    Shengjie Ling

Bibliographic Information

  • Book Title: Fibrous Proteins

  • Book Subtitle: Design, Synthesis, and Assembly

  • Editors: Shengjie Ling

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1574-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1573-7Published: 02 September 2021

  • Softcover ISBN: 978-1-0716-1576-8Published: 03 September 2022

  • eBook ISBN: 978-1-0716-1574-4Published: 01 September 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 264

  • Number of Illustrations: 17 b/w illustrations, 62 illustrations in colour

  • Topics: Chemistry/Food Science, general, Biotechnology, Biomaterials, Protein Science

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