Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2308)
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About this book
This volume brings together classical and cutting-edge protocols on the spatio-temporal study of the cellular subsets constituting the bone and the marrow in both mouse and human. Chapters details methods on bone marrow (BM) ecosystem, to label, sort, analyse, and culture specific cell subsets as well as techniques allowing the evaluation of the function of some of the cellular elements of the BM. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Bone Marrow Environment: Methods and Protocols aims to help new investigators to pursue the characterization of the BM microenvironment in the coming years.
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Keywords
Table of contents (22 protocols)
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Cell Culture and In Vitro Functionnal Assays
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Flow Cytometry-Based Analysis of the Bone Marrow Cellular Compartments
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Imaging of the Bone Marrow
Editors and Affiliations
Bibliographic Information
Book Title: Bone Marrow Environment
Book Subtitle: Methods and Protocols
Editors: Marion Espéli, Karl Balabanian
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-1425-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021
Hardcover ISBN: 978-1-0716-1424-2Published: 01 June 2021
Softcover ISBN: 978-1-0716-1427-3Published: 02 June 2022
eBook ISBN: 978-1-0716-1425-9Published: 31 May 2021
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 340
Number of Illustrations: 4 b/w illustrations, 67 illustrations in colour
Topics: Biotechnology, Stem Cells