Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2271)
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About this book
This volume presents methods used for the analysis of glycoproteins at different levels—intact, subunit, glycopeptide, and monosaccharide--, and discusses and solves most analytical challenges that a scientist working on glycoproteins may come across. The chapters in this book cover topics such as the role of glycosylation on the properties of therapeutic glycoproteins; different analytical methods to characterize glycosylation, from the intact proteins to the glycan level, for both N-linked and O-linked glycoproteins; mass spectrometry imaging methodology for glycosylation analysis in tissues; approaches to characterizing glycosylation on cultured cells; and the use of cloud computing to deploy mass spectrometry data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Mass Spectrometry of Glycoproteins: Methods and Protocols is a valuable resource for scientists interested in learning more about this developing field.
Cutting-edge and thorough, Mass Spectrometry of Glycoproteins: Methods and Protocols is a valuable resource for scientists interested in learning more about this developing field.
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Keywords
Table of contents (26 protocols)
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Front Matter
Editors and Affiliations
Bibliographic Information
Book Title: Mass Spectrometry of Glycoproteins
Book Subtitle: Methods and Protocols
Editors: Arnaud Delobel
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-1241-5
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021
Hardcover ISBN: 978-1-0716-1240-8Published: 29 April 2021
Softcover ISBN: 978-1-0716-1243-9Published: 28 April 2021
eBook ISBN: 978-1-0716-1241-5Published: 28 April 2021
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 401
Number of Illustrations: 14 b/w illustrations, 126 illustrations in colour