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Ribosome Profiling

Methods and Protocols

  • Includes cutting-edge techniques

  • Provides step-by-step detail essential for reproducible results

  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2252)

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eBook USD 119.00
Price excludes VAT (USA)
  • ISBN: 978-1-0716-1150-0
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 159.99
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
  2. Experimental Protocols

    1. Front Matter

      Pages 1-1
    2. Identification of Translation Start Sites in Bacterial Genomes

      • Sezen Meydan, Dorota Klepacki, Alexander S. Mankin, Nora Vázquez-Laslop
      Pages 27-55
    3. Genome-Wide Analysis of Translation in Replicatively Aged Yeast

      • Hanna Barlit, Manish K. Rai, Sara I. Shoushtari, Carine Beaupere, Vyacheslav M. Labunskyy
      Pages 151-173
    4. Tissue-Specific Ribosome Profiling in Drosophila

      • Xun Chen, Dion Dickman
      Pages 175-188
    5. Measuring Organ-Specific Translation Elongation Rate in Mice

      • Maxim V. Gerashchenko, Vadim N. Gladyshev
      Pages 189-200
    6. Active Ribosome Profiling with RiboLace: From Bench to Data Analysis

      • Massimiliano Clamer, Fabio Lauria, Toma Tebaldi, Gabriella Viero
      Pages 201-220
  3. Bioinformatics and Statistical Analysis

    1. Front Matter

      Pages 249-249
    2. Codon Resolution Analysis of Ribosome Profiling Data

      • Alexander Bartholomäus, Zoya Ignatova
      Pages 251-268
    3. Assessing Ribosome Distribution Along Transcripts with Polarity Scores and Regression Slope Estimates

      • Ilya E. Vorontsov, Artyom A. Egorov, Aleksandra S. Anisimova, Irina A. Eliseeva, Vsevolod J. Makeev, Vadim N. Gladyshev et al.
      Pages 269-294
    4. Genome-Wide Analysis of Actively Translated Open Reading Frames Using RiboTaper/ORFquant

      • Dermot Harnett, Eelco Meerdink, Lorenzo Calviello, Dominique Sydow, Uwe Ohler
      Pages 331-346
  4. Back Matter

    Pages 347-348

About this book

This book provides a single-source reference on the current state of the ribosome profiling method by describing experimental protocols for the quantitative analysis of translation in a variety of model organisms. In addition, the volume presents a detailed overview of the existing software tools and includes detailed description of methods for statistical analysis, data processing, and visualization of ribosome profiling data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Ribosome Profiling: Methods and Protocols aims to provide the type of standardized protocols that have previously been unavailable in an effort to bypass the major barriers to wide use of ribosome profiling-based approaches.

Keywords

  • Protein translation
  • Ribosomes
  • Genome-wide analysis
  • Software tools
  • Gene expression
  • RNA

Editors and Affiliations

  • Department of Dermatology, Boston University School of Medicine, Boston, USA

    Vyacheslav M. Labunskyy

Bibliographic Information

  • Book Title: Ribosome Profiling

  • Book Subtitle: Methods and Protocols

  • Editors: Vyacheslav M. Labunskyy

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1150-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1149-4

  • Softcover ISBN: 978-1-0716-1152-4

  • eBook ISBN: 978-1-0716-1150-0

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 348

  • Number of Illustrations: 19 b/w illustrations, 53 illustrations in colour

  • Topics: Biochemistry, Protein Biochemistry, Bioinformatics

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • ISBN: 978-1-0716-1150-0
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 159.99
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)