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The Integrin Interactome

Methods and Protocols

  • Book
  • © 2021

Overview

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2217)

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About this book

This volume provides the most cutting edge technologies related to the study of integrin activation and the characterization of their vast interactomes. Chapters detail protocols on experimental approached to quantify focal adhesion parameters, integrin activation, and the lateral interaction of integrins with transmembrane binding partners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, The Integrin Interactome: Methods and Protocols aims to give the reader a multi-scale journey from single bonds inside protein structures to the function of these crucial adhesion receptors at a whole organism level in physiology and pathology.


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Keywords

Table of contents (17 protocols)

  1. Integrin Activation and Focal Adhesion Measurements

  2. Proximity and Microscopy-Based Methods to Determine Integrin Interactions

  3. Biochemical, Proteomics and Computational Methods to Determine Integrin Interactions

  4. Biophysical Methods to Determine Integrin Activation and Its Cellular and Molecular Effects

  5. Integrin Activation and Interactions in Specific Systems and Processes

Editors and Affiliations

  • Molecular Mechanisms Program, Centro de Investigación del Cáncer and Instituto de Biología Molecular y Celular del Cáncer, Consejo Superior de Investigaciones Cientóficas (CSIC)-University of Salamanca, Salamanca, Spain

    Miguel Vicente-Manzanares

Bibliographic Information

  • Book Title: The Integrin Interactome

  • Book Subtitle: Methods and Protocols

  • Editors: Miguel Vicente-Manzanares

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0962-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0961-3Published: 20 November 2020

  • Softcover ISBN: 978-1-0716-0964-4Published: 20 November 2021

  • eBook ISBN: 978-1-0716-0962-0Published: 19 November 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIX, 315

  • Number of Illustrations: 11 b/w illustrations, 43 illustrations in colour

  • Topics: Cell Biology

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