Table of contents

  1. Front Matter
    Pages i-xi
  2. Vector Design for Vaccination

  3. DNA Vaccine Adjuvants and Immunostimulatory Response

    1. Front Matter
      Pages 49-49
    2. Neslihan Kayraklioglu, Begum Horuluoglu, Dennis M. Klinman
      Pages 51-85
  4. Biotechnological Processes to Obtain DNA Vaccines

    1. Front Matter
      Pages 133-133
    2. Alexandra Wagner, A. Rita Silva-Santos, Sara Sousa Rosa, Sophie Gierak, Ana M. Azevedo, Duarte Miguel F. Prazeres
      Pages 151-165
    3. A. Rita Silva-Santos, Sara Sousa Rosa, Duarte Miguel F. Prazeres, Ana M. Azevedo
      Pages 193-205
    4. Ana M. Almeida, Dalinda Eusébio, João A. Queiroz, Fani Sousa, Ângela Sousa
      Pages 207-222
  5. DNA Vaccine Delivery

    1. Front Matter
      Pages 223-223
    2. Pablo Cáceres-Morgado, Alvaro Lladser
      Pages 225-239
    3. Meihua Yu, Janin Chandra
      Pages 241-252
    4. Lucimara Gaziola de la Torre, Amanda Costa Silva Noronha Pessoa, Bruna Gregatti de Carvalho, Thiago Bezerra Taketa, Ismail Eş, Gabriel Perli
      Pages 253-269
    5. Diana Costa, Ângela Sousa, Rúben Faria, Ana Raquel Neves, João A. Queiroz
      Pages 271-284
    6. Nina D. Coelho-Rocha, Fernanda A. L. Barroso, Laísa M. Tavares, Ester S. S. dos Santos, Vasco Azevedo, Mariana M. Drumond et al.
      Pages 285-304
  6. DNA Vaccine Transfer to Clinical Trials

    1. Front Matter
      Pages 305-305
    2. Ana Cristina Ramalhinho, Miguel Castelo-Branco
      Pages 307-316
    3. Ana Cristina Ramalhinho, Miguel Castelo-Branco
      Pages 317-330
  7. Back Matter
    Pages 331-335

About this book


This volume details practical procedures on the latest DNA vaccine technology. Chapters guide readers through methods and protocols on DNA vaccine design, the adjuvant influence, production and purification methodologies, delivery systems, and approaches of the influence of DNA vaccines in the immunological response performance and in the cancer immunotherapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, DNA Vaccines: Methods and Protocols aims to ensure successful results in the further study of this vital field.


immunostimulatory immunotherapeutic bacterial vector Electroporation Liposomes Alzheimer’s Disease

Editors and affiliations

  • Ângela Sousa
    • 1
  1. 1.CICS-UBI – Health Sciences Research CentreUniversity of Beira InteriorCovilhãPortugal

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2021
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0871-5
  • Online ISBN 978-1-0716-0872-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site