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Leukemia Stem Cells

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. César Cobaleda

    1. Immune System Development and Function Unit, Centro de Biologia Molecular "Severo Ochoa", (CSIC/UAM), Madrid, Spain

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  2. Isidro Sánchez-García

    1. IBMCC, Laboratory 13, CSIC/Universidad de Salamanca, Salamanca, Spain

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2185)

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Table of contents (28 protocols)

  1. Protocols

    1. Lentiviral Transduction for Optimal LSC/HSC Manipulation

      • Gustavo Mostoslavsky
      Pages 299-306

    2. Characterizing the In Vivo Role of Candidate Leukemia Stem Cell Genes

      • Yu Wei Zhang, Julian Mess, Nina Cabezas-Wallscheid
      Pages 307-316

    3. Clonal Analysis of Patient-Derived Samples Using Cellular Barcodes

      • Sabrina Jacobs, Leonid V. Bystrykh, Mirjam E. Belderbos
      Pages 317-344

    4. Arrayed Molecular Barcoding of Leukemic Stem Cells

      • Marion Chapellier, Marcus Järås
      Pages 345-359

    5. In Vivo Generation of Leukemic Stem Cells by HSC Targeting by Transgenesis

      • Carolina Vicente-Dueñas
      Pages 361-372

    6. In Situ Hematopoietic Stem Cell Imaging

      • Aparna Venkatraman, Sarah E. Smith, Sandra Pinho, Meng Zhao, Linheng Li, Paul Frenette
      Pages 373-382

    7. A Genome Editing System for Therapeutical Targeting of Stem Cells

      • Giacomo Frati, Annarita Miccio
      Pages 383-398

    8. Method for the Generation of Induced Hematopoietic Stem Cells

      • Leonid Olender, Klil Levy, Roi Gazit
      Pages 399-410

    9. Modeling Leukemia Stem Cells with Patient-Derived Induced Pluripotent Stem Cells

      • André G. Deslauriers, Andriana G. Kotini, Eirini P. Papapetrou
      Pages 411-422

    10. High-Content Imaging to Phenotype Human Primary and iPSC-Derived Cells

      • Lorenzo Veschini, Heba Sailem, Disha Malani, Vilja Pietiäinen, Ana Stojiljkovic, Erika Wiseman et al.
      Pages 423-445

    11. Bioinformatic Methods to Identify Mutational Signatures in Cancer

      • S. M. Ashiqul Islam, Ludmil B. Alexandrov
      Pages 447-473

  2. Back Matter

    Pages 475-479
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Keywords

About this book

The detailed volume aims to provide a comprehensive hands-on manual covering all the techniques involved in the cellular and molecular identification and characterization of both normal hematopoietic and leukemic stem cells, both from human patients and from mouse models of human leukemia. The book also covers the most frequently used experimental approaches for the generation of such stem cell-based models of human leukemia. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Leukemia Stem Cells: Methods and Protocols serves as an ideal guide for researchers, both expert and novice, seeking to further our knowledge of this vital avenue of cancer research.

Editors and Affiliations

  • Immune System Development and Function Unit, Centro de Biologia Molecular "Severo Ochoa", (CSIC/UAM), Madrid, Spain

    César Cobaleda

  • IBMCC, Laboratory 13, CSIC/Universidad de Salamanca, Salamanca, Spain

    Isidro Sánchez-García

Bibliographic Information

  • Book Title: Leukemia Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: César Cobaleda, Isidro Sánchez-García

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0810-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0809-8Published: 10 November 2020

  • Softcover ISBN: 978-1-0716-0812-8Published: 10 November 2021

  • eBook ISBN: 978-1-0716-0810-4Published: 09 November 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 479

  • Number of Illustrations: 16 b/w illustrations, 71 illustrations in colour

  • Topics: Cancer Research, Stem Cells, Biomedicine, general, Pharmacology/Toxicology

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