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Leukemia Stem Cells

Methods and Protocols

  • Includes cutting-edge techniques

  • Provides step-by-step detail essential for reproducible results

  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2185)

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • ISBN: 978-1-0716-0810-4
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 159.99
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)

This is a preview of subscription content, access via your institution.

Table of contents (28 protocols)

  1. Front Matter

    Pages i-xiii
  2. Introductory Reviews

    1. Front Matter

      Pages 1-1
    2. Leukemia Stem Cells: Concept and Implications

      • Isidro Sánchez-García, César Cobaleda
      Pages 25-37
    3. Leukemia Stem Cell Drug Discovery

      • César Cobaleda, Isidro Sánchez-García
      Pages 39-48
  3. Protocols

    1. Front Matter

      Pages 49-49
    2. Analysis and Isolation of Mouse Leukemic Stem Cells

      • Fang Dong, Haitao Bai, Hideo Ema
      Pages 51-63
    3. Mass Cytometry of Hematopoietic Cells

      • Astraea Jager, Jolanda Sarno, Kara L. Davis
      Pages 65-76
    4. PCR Technology to Identify Minimal Residual Disease

      • Giovanni Cazzaniga, Simona Songia, Andrea Biondi, On Behalf of the EuroMRD Working Group
      Pages 77-94
    5. Next-Generation Sequencing Technology to Identify Minimal Residual Disease in Lymphoid Malignancies

      • Michaela Kotrova, Nikos Darzentas, Christiane Pott, Monika Brüggemann, On Behalf of the EuroClonality-NGS Working Group
      Pages 95-111
    6. Genomic Inverse PCR for Screening of Preleukemic Cells in Newborns (GIPFEL Technology)

      • Daniel Hein, Arndt Borkhardt, Ute Fischer
      Pages 113-134
    7. Single-Cell Transcriptomic Analysis of Hematopoietic Cells

      • Paulina M. Strzelecka, Anna M. Ranzoni, Ana Cvejic
      Pages 135-158
    8. In-Depth Mass Spectrometry-Based Single-Cell and Nanoscale Proteomics

      • Yiran Liang, Thy Truong, Ying Zhu, Ryan T. Kelly
      Pages 159-179
    9. Experimental Competitive Bone Marrow Transplant Assays

      • Roxann Hétu-Arbour, Sarah Bouali, Krista M. Heinonen
      Pages 195-214
    10. Human T-ALL Xenografts

      • Patricia Fuentes, María L. Toribio, Sara González-García
      Pages 215-239
    11. An Experimental and Computational Protocol to Study Cell Proliferation in Human Acute Myeloid Leukemia Xenografts

      • Thalia Vlachou, Marco S. Nobile, Chiara Ronchini, Daniela Besozzi, Pier Giuseppe Pelicci
      Pages 241-258
    12. Leukemic Stem Cell Culture in Cytokine-Free Medium

      • Xiaolei Liu, Peter S. Klein
      Pages 259-265
    13. Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acid

      • Luena Papa, Mansour Djedaini, Manisha Kintali, Christoph Schaniel, Ronald Hoffman
      Pages 267-280
    14. Isolation, Culture, and Manipulation of Human Cord Blood Progenitors

      • Cristina Prieto, Damia Romero-Moya, Rosa Montes
      Pages 281-298

About this book

The detailed volume aims to provide a comprehensive hands-on manual covering all the techniques involved in the cellular and molecular identification and characterization of both normal hematopoietic and leukemic stem cells, both from human patients and from mouse models of human leukemia. The book also covers the most frequently used experimental approaches for the generation of such stem cell-based models of human leukemia. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Leukemia Stem Cells: Methods and Protocols serves as an ideal guide for researchers, both expert and novice, seeking to further our knowledge of this vital avenue of cancer research.

Keywords

  • Cancer stem cells
  • Hematopoietic stem cells
  • Tissue culture
  • LSCs
  • Human leukemias
  • Animal models

Editors and Affiliations

  • Immune System Development and Function Unit, Centro de Biologia Molecular "Severo Ochoa", (CSIC/UAM), Madrid, Spain

    César Cobaleda

  • IBMCC, Laboratory 13, CSIC/Universidad de Salamanca, Salamanca, Spain

    Isidro Sánchez-García

Bibliographic Information

  • Book Title: Leukemia Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: César Cobaleda, Isidro Sánchez-García

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0810-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0809-8

  • Softcover ISBN: 978-1-0716-0812-8

  • eBook ISBN: 978-1-0716-0810-4

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 479

  • Number of Illustrations: 16 b/w illustrations, 71 illustrations in colour

  • Topics: Cancer Biology, Stem Cell Biology, Biomedical Research, Pharmacology

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • ISBN: 978-1-0716-0810-4
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 159.99
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)