Overview
- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2180)
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About this book
This fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and authoritative, Cryopreservation and Freeze-Drying Protocols, Fourth Edition serves as an ideal guide for researchers and technical assistants in academia and industry with a background in life sciences, medicine, or engineering who want to investigate freezing and drying of biological systems or set up methods to safely store biological specimens while maintaining their function upon reconstitution.
The chapter “Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Thorough and authoritative, Cryopreservation and Freeze-Drying Protocols, Fourth Edition serves as an ideal guide for researchers and technical assistants in academia and industry with a background in life sciences, medicine, or engineering who want to investigate freezing and drying of biological systems or set up methods to safely store biological specimens while maintaining their function upon reconstitution.
The chapter “Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation” is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
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Keywords
Table of contents (41 protocols)
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Front Matter
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Fundamental Aspects of Cryopreservation and Freeze-Drying
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Front Matter
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Technologies and Methods to Study Freezing and Drying
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Front Matter
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Cryopreservation and Freeze-Drying Protocols
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Front Matter
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Editors and Affiliations
Bibliographic Information
Book Title: Cryopreservation and Freeze-Drying Protocols
Editors: Willem F. Wolkers, Harriëtte Oldenhof
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-0783-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021
Hardcover ISBN: 978-1-0716-0782-4Published: 15 August 2020
Softcover ISBN: 978-1-0716-0785-5Published: 12 May 2022
eBook ISBN: 978-1-0716-0783-1Published: 14 August 2020
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 4
Number of Pages: XVI, 742
Number of Illustrations: 52 b/w illustrations, 104 illustrations in colour
Topics: Biotechnology