© 2021

Cryopreservation and Freeze-Drying Protocols

  • Willem F. Wolkers
  • Harriëtte Oldenhof
  • Includes cutting-edge techniques

  • Provides step-by-step detail essential for reproducible results

  • Contains key implementation advice from the experts


Part of the Methods in Molecular Biology book series (MIMB, volume 2180)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Fundamental Aspects of Cryopreservation and Freeze-Drying

    1. Front Matter
      Pages 1-1
    2. Willem F. Wolkers, Harriëtte Oldenhof
      Pages 3-25
    3. Gregory M. Fahy, Brian Wowk
      Pages 27-97
    4. Ross M. Warner, Adam Z. Higgins
      Pages 173-188
  3. Technologies and Methods to Study Freezing and Drying

    1. Front Matter
      Pages 189-189
    2. Peter Kilbride, Julie Meneghel
      Pages 191-201 Open Access
    3. Shangping Wang, Susan Trammell, Gloria D. Elliott
      Pages 203-220
    4. Kezhou Wu, Leila Laouar, Nadia Shardt, Janet A. W. Elliott, Nadr M. Jomha
      Pages 303-315
    5. Ariadna Corral, Alberto Olmo, Ramón Risco
      Pages 317-330
    6. Willem F. Wolkers, Harriëtte Oldenhof
      Pages 331-349
  4. Cryopreservation and Freeze-Drying Protocols

    1. Front Matter
      Pages 363-363
    2. Harriëtte Oldenhof, Willem F. Wolkers, Harald Sieme
      Pages 365-377
    3. Henri Woelders
      Pages 379-399

About this book


This fourth edition explores fully up-to-date standardly used cryopreservation, vitrification, and freeze-drying protocols for specimens that are used for research purposes, conservation of genetic reserves, and applications in agriculture and medicine. Beginning with a section on the fundamentals as well as the use of mathematical modeling to solve cryobiological problems, the book continues with sections on technological aspects of freezing and drying, analytical methods to study protectant loading of cells and tissues, cell behavior during freezing and drying, and thermodynamic properties of preservation solutions, as well as cryopreservation, vitrification, and freeze-drying protocols for a wide variety of samples and different applications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Thorough and authoritative, Cryopreservation and Freeze-Drying Protocols, Fourth Edition serves as an ideal guide for researchers and technical assistants in academia and industry with a background in life sciences, medicine, or engineering who want to investigate freezing and drying of biological systems or set up methods to safely store biological specimens while maintaining their function upon reconstitution.

The chapter “Freezing Technology: Control of Freezing, Thawing, and Ice Nucleation” is available open access under a Creative Commons Attribution 4.0 International License via


Cryobiology Vitrification Cryostorage Freeze-dried specimens Human samples Animal samples Plant samples

Editors and affiliations

  • Willem F. Wolkers
    • 1
  • Harriëtte Oldenhof
    • 2
  1. 1.Unit for Reproductive Medicine—Clinic for Horses, Biostabilization Laboratory—Lower Saxony Centre for Biomedical Engineering, Implant Research and Development, University of Veterinary Medicine Hannover, Hannover, GermanyUniversity of Veterinary Medicine HannoverHannoverGermany
  2. 2.Unit for Reproductive Medicine—Clinic for HorsesUniversity of Veterinary Medicine HannoverHannoverGermany

Bibliographic information

  • Book Title Cryopreservation and Freeze-Drying Protocols
  • Editors Willem F. Wolkers
    Harriëtte Oldenhof
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2021
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-0716-0782-4
  • Softcover ISBN 978-1-0716-0785-5
  • eBook ISBN 978-1-0716-0783-1
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 4
  • Number of Pages XVI, 742
  • Number of Illustrations 52 b/w illustrations, 104 illustrations in colour
  • Topics Biotechnology
  • Buy this book on publisher's site