Skip to main content
  • Book
  • © 2021

Protein Downstream Processing

Design, Development, and Application of High and Low-Resolution Methods

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2178)

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • ISBN: 978-1-0716-0775-6
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 199.99
Price excludes VAT (USA)
Hardcover Book USD 279.99
Price excludes VAT (USA)

This is a preview of subscription content, access via your institution.

Table of contents (30 protocols)

  1. High-Resolution Protein Purification Methods

    1. Macroporous Polymer Monoliths for Affinity Chromatography and Solid-Phase Enzyme Processing

      • E. G. Korzhikova-Vlakh, G. A. Platonova, T. B. Tennikova
      Pages 251-284
    2. Sample Displacement Batch Chromatography of Proteins

      • Laura Heikaus, Siti Hidayah, Manasia Gaikwad, Marta Kotasinska, Verena Richter, Marcel Kwiatkowski et al.
      Pages 285-299
    3. Lectin Affinity Chromatography: An Efficient Method to Purify Horse IgG3

      • Salvatore G. De-Simone, David W. Provance Jr.
      Pages 301-310
    4. Heparin-Binding Affinity Tag: A Novel Affinity Tag for Simple and Efficient Purification of Recombinant Proteins

      • Sanhita Maity, Musaab Al-Ameer, Ravi Kumar Gundampati, Shilpi Agrawal, Thallapuranam Krishnaswamy Suresh Kumar
      Pages 311-328
    5. Expression and Purification of Recombinant Proteins in Escherichia coli Tagged with the Metal-Binding Proteins SmbP and CusF3H+

      • Jessica J. Gomez-Lugo, Bryan D. Santos, David A. Perez-Perez, Jorge M. Montfort-Gardeazabal, Megan M. McEvoy, Xristo Zarate
      Pages 329-344
    6. Antibody Aggregate Removal Using a Mixed-Mode Chromatography Resin

      • Tao Chen, Gaili Guo, Guoqing Tan, Ying Wang, Yifeng Li
      Pages 345-354
  2. Assessing Protein Structural Integrity, Purity, and Stabilization

    1. Front Matter

      Pages 355-355
    2. Proteomic Analysisof Food Allergens by MALDI TOF/TOF Mass Spectrometry

      • Cosima D. Calvano, Mariachiara Bianco, Ilario Losito, Tommaso R. I. Cataldi
      Pages 357-376
    3. Protein Structure Analysis and Validation with X-Ray Crystallography

      • Anastassios C. Papageorgiou, Nirmal Poudel, Jesse Mattsson
      Pages 377-404
    4. Stabilization of Proteins by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin

      • Pavel Dráber, Vadym Sulimenko, Tetyana Sulimenko, Eduarda Dráberová
      Pages 417-435
  3. Applications/Case Studies

    1. Front Matter

      Pages 437-437
    2. G-Protein-Coupled Receptor Expression and Purification

      • Karolina Corin, Lotta T. Tegler, Sotirios Koutsopoulos
      Pages 439-467
    3. (Hyper)Thermophilic Enzymes: Production and Purification

      • Pierpaolo Falcicchio, Mark Levisson, Servé W. M. Kengen, Sotirios Koutsopoulos, John van der Oost
      Pages 469-478
    4. Screening of Recombinant Lignocellulolytic Enzymes Through Rapid Plate Assays

      • Anthi Karnaouri, Anastasia Zerva, Paul Christakopoulos, Evangelos Topakas
      Pages 479-503
  4. Back Matter

    Pages 505-511

About this book

This second edition volume expands on the previous edition with updated research and techniques to help laboratory workers design and implement a successful purification strategy, emphasize critical aspects on practical problems, and answers questions encountered at the lab bench. The chapters in this book are divided into five parts: Part One discusses an overview of screening and design of purification strategies and covers initial aspects on high-throughput screening, methods development, and media selection; Parts Two and Three explore low- and high-resolution methods, with emphasis on affinity chromatography; Part Four describes analytical techniques of purified proteins; and Part Five presents selected examples and case studies to discuss the aforementioned. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and comprehensive, Protein Downstream Processing: Design, Development, and Application of High and Low-Resolution Methods, Second Edition is an ideal source of information to advanced students, junior researchers, and scientists involved in health sciences, cellular and molecular biology, biochemistry, biotechnology, and other related areas in both academia and industry. 

Keywords

  • Process Chromatography
  • Membrane Chromatography
  • ion exchange
  • dye-ligands
  • microscale chromatography pipette tips
  • bioinformatics
  • Two-Phase Systems

Editors and Affiliations

  • Laboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, Athens, Greece

    Nikolaos E. Labrou

Bibliographic Information

  • Book Title: Protein Downstream Processing

  • Book Subtitle: Design, Development, and Application of High and Low-Resolution Methods

  • Editors: Nikolaos E. Labrou

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0775-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-0774-9

  • Softcover ISBN: 978-1-0716-0777-0

  • eBook ISBN: 978-1-0716-0775-6

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XV, 511

  • Number of Illustrations: 49 b/w illustrations, 78 illustrations in colour

  • Topics: Protein Biochemistry, Chemistry, Biotechnology, Biomaterials

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • ISBN: 978-1-0716-0775-6
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 199.99
Price excludes VAT (USA)
Hardcover Book USD 279.99
Price excludes VAT (USA)