Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2169)
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Table of contents (18 protocols)
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Front Matter
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Back Matter
Keywords
About this book
This volume explores techniques used to study either the structure or the functions of caveolae and their components in several normal and pathophysiological situations. The chapters in this book cover topics such as selective visualization of caveolae by electron microscopy techniques; spatiotemporal analysis of caveolae dynamics and mechanics using live cell fluorescence microscopy; in vitro reconstitution such as liposomes and GPMVs as tools to study caveolin- and cavin-interacting partners; pulling of tethers from the cell plasma membrane using optical tweezers; and immunofluorescence-based analysis of caveolin-3 in the diagnostic management of neuromuscular diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and comprehensive, Caveolae: Methods and Protocols is a valuable resource for both novice and expert researchers who are interested in discovering new roles or regulations of formed caveolae, and proteins composing their coat in various model organisms.
Editors and Affiliations
Bibliographic Information
Book Title: Caveolae
Book Subtitle: Methods and Protocols
Editors: Cedric M. Blouin
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-0732-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020
Hardcover ISBN: 978-1-0716-0731-2Published: 17 June 2020
Softcover ISBN: 978-1-0716-0734-3Published: 17 June 2021
eBook ISBN: 978-1-0716-0732-9Published: 16 June 2020
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 218
Number of Illustrations: 9 b/w illustrations, 37 illustrations in colour
Topics: Lipidology, Cell Biology