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  • © 2020

RNA Tagging

Methods and Protocols

Editors:

  1. Manfred Heinlein

    1. Institut de Biologie Moléculaire des Plantes (IBMP-CNRS), Université de Strasbourg, Strasbourg, France

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  • Includes cutting-edge techniques

  • Provides step-by-step detail essential for reproducible results

  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2166)

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  • ISBN: 978-1-0716-0712-1
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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xiv
    PDF

  2. In Situ Detection of RNA Molecules Using FISH Techniques

    1. Front Matter

      Pages 1-1
      PDF

    3. Quantitative Fluorescence In Situ Hybridization Detection of Plant mRNAs with Single-Molecule Resolution

      • Kun Huang, Mona Batish, Chong Teng, Alex Harkess, Blake C. Meyers, Jeffrey L. Caplan
      Pages 23-33

    4. Visualization of Endoplasmic Reticulum-Associated mRNA in Mammalian Cells

      • Jingze J. Wu, Alexander F. Palazzo
      Pages 35-49

  3. In Vivo Imaging of RNA Transport and Localization

    1. Front Matter

      Pages 71-71
      PDF

    3. Visualization of Transiently Expressed mRNA in Plants Using MS2

      • Eduardo José Peña, Manfred Heinlein
      Pages 103-120

    4. New Generations of MS2 Variants and MCP Fusions to Detect Single mRNAs in Living Eukaryotic Cells

      • Xavier Pichon, Marie-Cécile Robert, Edouard Bertrand, Robert H. Singer, Evelina Tutucci
      Pages 121-144

    5. In Vivo Imaging of Mobile mRNAs in Plants Using MS2

      • Kai-Ren Luo, Nien-Chen Huang, Tien-Shin Yu
      Pages 145-155

    6. RNA Imaging with RNase-Inactivated Csy4 in Plants and Filamentous Fungi

      • David Burnett, Alexander Lichius, Jens Tilsner
      Pages 157-178

  4. Imaging and Analysis of RNA Uptake and Transport Between Cells

    1. Front Matter

      Pages 179-179
      PDF

    2. Utilizing Potato Virus X to Monitor RNA Movement

      • Zhiming Yu, Sung Ki Cho, Pengcheng Zhang, Yiguo Hong, David J. Hannapel
      Pages 181-194

    4. Synthesizing Fluorescently Labeled dsRNAs and sRNAs to Visualize Fungal RNA Uptake

      • Rachael Hamby, Ming Wang, Lulu Qiao, Hailing Jin
      Pages 215-225

    5. Labeling of dsRNA for Fungal Uptake Detection Analysis

      • Matteo Galli, Jafargholi Imani, Karl-Heinz Kogel
      Pages 227-238

  5. Identification and Analysis of RNA-Binding Proteins

    1. Front Matter

      Pages 239-239
      PDF
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About this book

This book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, and disease. Beginning with a section on in situ detection of RNA molecules using FISH techniques, the volume continues with parts exploring in vivo imaging of RNA transport and localization, imaging and analysis of RNA uptake and transport between cells, identification and analysis of RNA-binding proteins, guide RNAs in genome editing, as well as other specific analytical techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, RNA Tagging: Methods and Protocols serves as a vital reference for researchers looking to further the increasingly important research in RNA biology.
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Keywords

  • RNA-binding proteins
  • RNA transport
  • RNA-mediated editing
  • CRISPR
  • Guide RNA design
  • Functional RNA complexes
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Editors and Affiliations

  • Institut de Biologie Moléculaire des Plantes (IBMP-CNRS), Université de Strasbourg, Strasbourg, France

    Manfred Heinlein

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Bibliographic Information

  • Book Title: RNA Tagging

  • Book Subtitle: Methods and Protocols

  • Editors: Manfred Heinlein

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0712-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0711-4Published: 25 July 2020

  • Softcover ISBN: 978-1-0716-0714-5Published: 26 July 2021

  • eBook ISBN: 978-1-0716-0712-1Published: 24 July 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 490

  • Number of Illustrations: 14 b/w illustrations, 77 illustrations in colour

  • Topics: Protein Biochemistry, Biochemistry

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Access via your institution
eBook USD 119.00
Price excludes VAT (USA)
  • ISBN: 978-1-0716-0712-1
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 159.99
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)
Learn about institutional subscriptions