RNA Tagging

Methods and Protocols

  • Manfred Heinlein

Part of the Methods in Molecular Biology book series (MIMB, volume 2166)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. In Situ Detection of RNA Molecules Using FISH Techniques

  3. In Vivo Imaging of RNA Transport and Localization

    1. Front Matter
      Pages 71-71
    2. Eduardo José Peña, Manfred Heinlein
      Pages 103-120
    3. Xavier Pichon, Marie-Cécile Robert, Edouard Bertrand, Robert H. Singer, Evelina Tutucci
      Pages 121-144
    4. Kai-Ren Luo, Nien-Chen Huang, Tien-Shin Yu
      Pages 145-155
    5. David Burnett, Alexander Lichius, Jens Tilsner
      Pages 157-178
  4. Imaging and Analysis of RNA Uptake and Transport Between Cells

    1. Front Matter
      Pages 179-179
    2. Zhiming Yu, Sung Ki Cho, Pengcheng Zhang, Yiguo Hong, David J. Hannapel
      Pages 181-194
    3. Rachael Hamby, Ming Wang, Lulu Qiao, Hailing Jin
      Pages 215-225
    4. Matteo Galli, Jafargholi Imani, Karl-Heinz Kogel
      Pages 227-238
  5. Identification and Analysis of RNA-Binding Proteins

  6. RNAs as Guides for Genome Editing and Imaging of Chromosome Loci

    1. Front Matter
      Pages 329-329
    2. Patrick Schindele, Felix Wolter, Holger Puchta
      Pages 331-342
    3. Solmaz Khosravi, Steven Dreissig, Patrick Schindele, Felix Wolter, Twan Rutten, Holger Puchta et al.
      Pages 343-356
    4. Xiaotian Wu, Yachen Ying, Shiqi Mao, Christopher J. Krueger, Antony K. Chen
      Pages 357-372
  7. Specific Techniques for the Analysis of RNA Functions

    1. Front Matter
      Pages 385-385
    2. Marcel Böhrer, Bart Rymen, Christophe Himber, Aude Gerbaud, David Pflieger, Debbie Laudencia-Chingcuanco et al.
      Pages 387-411
    3. Stéphanie Lalande, Marjorie Chery, Elodie Ubrig, Guillaume Hummel, Julie Kubina, Angèle Geldreich et al.
      Pages 413-429
    4. Soledad Traubenik, Flavio Blanco, María Eugenia Zanetti, Mauricio A. Reynoso
      Pages 451-472
  8. Back Matter
    Pages 487-490

About this book


This book provides a compendium of state-of-the-art methods for the labeling, detection, and purification of RNA and RNA-protein complexes and thereby constitutes an important toolbox for researchers interested in understanding the complex roles of RNA molecules in development, signaling, and disease. Beginning with a section on in situ detection of RNA molecules using FISH techniques, the volume continues with parts exploring in vivo imaging of RNA transport and localization, imaging and analysis of RNA uptake and transport between cells, identification and analysis of RNA-binding proteins, guide RNAs in genome editing, as well as other specific analytical techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, RNA Tagging: Methods and Protocols serves as a vital reference for researchers looking to further the increasingly important research in RNA biology.


RNA-binding proteins RNA transport RNA-mediated editing CRISPR Guide RNA design Functional RNA complexes

Editors and affiliations

  • Manfred Heinlein
    • 1
  1. 1.Institut de Biologie Moléculaire des Plantes (IBMP-CNRS)Université de StrasbourgStrasbourgFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC, part of Springer Nature 2020
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-0716-0711-4
  • Online ISBN 978-1-0716-0712-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site