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Volume Microscopy

Multiscale Imaging with Photons, Electrons, and Ions

  • Book
  • © 2020

Overview

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 155)

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About this book

This volume discusses different approaches to workflows for large volume electron microscopy - from preparation of samples to their imaging in a variety of microscopes - in some cases also applying correlative techniques. The chapters in this book cover topics such as correlative super resolution and electron microscopy to detect molecules in their native cellular context; low-threshold access to serial section arrays; improving serial blockface SEM by focal charge compensation; FIBSEM analysis of interfaces between hard technical devices and soft neuronal tissue; and image processing for volume electron microscopy. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and authoritative, Volume Microscopy: Multiscale Imaging with Photons, Electrons, and Ions is a valuable resource for novice and expert scientists interested in learning more about this evolving field.

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Keywords

Table of contents (14 protocols)

Editors and Affiliations

  • Cryo EM, Centre for Advanced Materials (CAM), Universität Heidelberg, Heidelberg, Germany

    Irene Wacker

  • Sector Life Science, Carl Zeiss Microscopy GmbH, Oberkochen, Germany, Carl Zeiss, Oberkochen, Germany

    Eric Hummel

  • BioQuant, Universität Heidelberg, Heidelberg, Germany, Cryo EM, BioQuant, Universitätsklinikum Heidelberg, Heidelberg, Germany

    Steffen Burgold

  • Cryo EM, Centre for Advanced Materials (CAM), Universität Heidelberg, Heidelberg, Germany, Cryo EM, BioQuant, Universitätsklinikum Heidelberg, Heidelberg, Germany

    Rasmus Schröder

Bibliographic Information

  • Book Title: Volume Microscopy

  • Book Subtitle: Multiscale Imaging with Photons, Electrons, and Ions

  • Editors: Irene Wacker, Eric Hummel, Steffen Burgold, Rasmus Schröder

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-0716-0691-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0690-2Published: 30 June 2020

  • Softcover ISBN: 978-1-0716-0693-3Published: 05 July 2020

  • eBook ISBN: 978-1-0716-0691-9Published: 29 June 2020

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XVII, 306

  • Number of Illustrations: 19 b/w illustrations, 88 illustrations in colour

  • Topics: Neurosciences, Analytical Chemistry, Materials Science, general

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