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Pollen and Pollen Tube Biology

Methods and Protocols

Editors:

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  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2160)

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eBook
USD 119.00
Price excludes VAT (USA)
  • ISBN: 978-1-0716-0672-8
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  • Readable on all devices
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  • Tax calculation will be finalised during checkout
Softcover Book
USD 159.99
Price excludes VAT (USA)
Hardcover Book
USD 219.99
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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiii
  2. Pollen Grain Counting Using a Cell Counter

    • Hiroyuki Kakui, Misako Yamazaki, Naoto-Benjamin Hamaya, Kentaro K. Shimizu
    Pages 1-11
  3. Isolation of the Pistil-Stimulated Pollen Tube Secretome

    • Said Hafidh, David Honys
    Pages 41-72
  4. Restricted Pollination for Tracing Individual Pollen Tubes in a Pistil

    • Taro Takahashi, Toshiyuki Mori, Tomoko Igawa
    Pages 73-81
  5. Semi-In Vivo Assay for Pollen Tube Attraction

    • Sheng Zhong, Zhijuan Wang, Li-Jia Qu
    Pages 83-92
  6. Workflow to Characterize Mutants with Reproductive Defects

    • Jennifer A. Noble, Ravishankar Palanivelu
    Pages 109-128
  7. Arabidopsis thaliana Pollen Tube Culture for Multi-omics Studies

    • Mario Costa, Jessy Silva, Silvia Coimbra
    Pages 149-165
  8. Obtaining Mutant Pollen for Phenotypic Analysis and Pollen Tube Dual Staining

    • Sheng Zhong, Zhijuan Wang, Li-Jia Qu
    Pages 181-190
  9. Analyzing Intracellular Gradients in Pollen Tubes

    • Daniel S. C. Damineli, Maria Teresa Portes, José A. Feijó
    Pages 201-210
  10. Silicone Chambers for Pollen Tube Imaging in Microstructured In Vitro Environments

    • Hana Bertrand-Rakusová, Youssef Chebli, Anja Geitmann
    Pages 211-221
  11. Imaging and Analysis of the Content of Callose, Pectin, and Cellulose in the Cell Wall of Arabidopsis Pollen Tubes Grown In Vitro

    • Ana R. Sede, Diego L. Wengier, Cecilia Borassi, José M. Estevez, Jorge P. Muschietti
    Pages 233-242

About this book

This volume explores a collection of experimental techniques used to investigate different aspects of pollen development and function, including its role in reaching the ovule and delivering the two sperm cells. The techniques discussed range from basic methodology to cultivate pollen in vitro to the sophisticated experiments involving micromanipulation, Lab-on-Chip technology, or high-end imaging. The chapters in this book cover topics such as pollen grain counting using a cell counter; restricted pollination for tracing individual pollen tubes in a pistil; obtaining mutant pollen for phenotypic analysis and pollen tube dual-staining; analyzing intracellular gradients in pollen tubes; and measuring exocytosis rate in Arabidopsis pollen tube using corrected fluorescence recovery after photoconversion (cFRAPc) technique. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Pollen and Pollen Tube Biology: Methods and Protocols is a valuable resource for novice and expert scientists interested in learning more about the field of plant reproduction. 

Keywords

  • gametophyte
  • Bimolecular fluorescence
  • Genome-wide association study (GWAS)
  • RStudio
  • RNA-Seq expression

Editors and Affiliations

  • Department of Plant Science, McGill University, Montreal, Canada

    Anja Geitmann

Bibliographic Information

  • Book Title: Pollen and Pollen Tube Biology

  • Book Subtitle: Methods and Protocols

  • Editors: Anja Geitmann

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0672-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0671-1

  • Softcover ISBN: 978-1-0716-0674-2

  • eBook ISBN: 978-1-0716-0672-8

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 332

  • Number of Illustrations: 7 b/w illustrations, 90 illustrations in colour

  • Topics: Plant Science

Buying options

eBook
USD 119.00
Price excludes VAT (USA)
  • ISBN: 978-1-0716-0672-8
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD 159.99
Price excludes VAT (USA)
Hardcover Book
USD 219.99
Price excludes VAT (USA)