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In Situ Hybridization Protocols

  • Book
  • © 2020
  • Latest edition

Overview

Editors:
  1. Boye Schnack Nielsen

    1. Molecular Histology, Bioneer A/S, Horsholm, Denmark

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    You can also search for this editor in PubMed   Google Scholar

  2. Julia Jones

    1. Cancer Research UK, University of Cambridge, Cambridge, UK

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains keynotes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2148)

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About this book

This fifth edition volume expands on the previous editions with updated discussions on the many new in situ hybridization (ISH) techniques used by researchers today. New developments in probe designs, detection systems, specificity and sensitivity improvements, and multiplexing combinations are explored. Chapters in this book are organized into seven sections and cover general applications; methods for DNA ISH; methods for cultured cells; methods for wholemount and plant material; automated methods for RNA; multiplexing and combined methods; and targeted selective methods and single molecule detections. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, In Situ Hybridization Protocols, Fifth Edition is a valuable resource for both novice and expert scientists interested in learning more about this exciting and advancing field. 


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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xiii
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  3. Methods for DNA ISH

    1. Front Matter

      Pages 33-33
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    2. Practical Application of Fluorescent In Situ Hybridization Techniques in Clinical Diagnostic Laboratories

      • Sheila J. M. O’Connor, Kathryn R. Turner, Sharon L. Barrans
      Pages 35-70

    3. Fluorescent In Situ Hybridization Using Oligonucleotide-Based Probes

      • Guilherme T. Braz, Fan Yu, Lívia do Vale Martins, Jiming Jiang
      Pages 71-83

    4. Visualizing Genome Reorganization Using 3D DNA FISH

      • Alasdair Jubb, Shelagh Boyle
      Pages 85-95

  4. Methods for Cultured Cells

    1. Front Matter

      Pages 97-97
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    2. MicroRNA In Situ Hybridization in Paraffin-Embedded Cultured Cells

      • Jaslin P. James, Laura Johnsen, Trine Møller, Boye Schnack Nielsen
      Pages 99-110

    3. Multiplexed Detection and Analysis of Low-Abundance Long Noncoding RNA Using RNAscope™ in Cultured Cells

      • Julia Jones, Heather Zecchini, Sankari Nagarajan
      Pages 111-125

    4. Multiplexed Quantitative In Situ Hybridization for Mammalian or Bacterial Cells in Suspension: qHCR Flow Cytometry (v3.0)

      • Maayan Schwarzkopf, Harry M. T. Choi, Niles A. Pierce
      Pages 127-141

    5. Multiplexed Quantitative In Situ Hybridization for Mammalian Cells on a Slide: qHCR and dHCR Imaging (v3.0)

      • Maayan Schwarzkopf, Harry M. T. Choi, Niles A. Pierce
      Pages 143-156

  5. Methods for Wholemounts and Plant Material

    1. Front Matter

      Pages 157-157
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    2. Multiplexed Quantitative In Situ Hybridization with Subcellular or Single-Molecule Resolution Within Whole-Mount Vertebrate Embryos: qHCR and dHCR Imaging (v3.0)

      • Harry M. T. Choi, Maayan Schwarzkopf, Niles A. Pierce
      Pages 159-178

    3. Hybridization Chain Reaction for Quantitative and Multiplex Imaging of Gene Expression in Amphioxus Embryos and Adult Tissues

      • Toby G. R. Andrews, Giacomo Gattoni, Lara Busby, Michael A. Schwimmer, Èlia Benito-Gutiérrez
      Pages 179-194

    4. RNAscope™ Multiplex Detection in Zebrafish

      • Theresa Gross-Thebing
      Pages 195-202

    5. Duplex In Situ Hybridization of Virus Nucleic Acids in Plant Tissues Using RNAscope®

      • Samar Sheat, Stephan Winter, Paolo Margaria
      Pages 203-215

  6. Automated Methods for RNA

    1. Front Matter

      Pages 217-217
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    2. Automated ISH for Validated Histological Mapping of Lowly Expressed Genes

      • Charles Pyke
      Pages 219-228
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Editors and Affiliations

  • Molecular Histology, Bioneer A/S, Horsholm, Denmark

    Boye Schnack Nielsen

  • Cancer Research UK, University of Cambridge, Cambridge, UK

    Julia Jones

Bibliographic Information

  • Book Title: In Situ Hybridization Protocols

  • Editors: Boye Schnack Nielsen, Julia Jones

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0623-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0622-3Published: 12 May 2020

  • Softcover ISBN: 978-1-0716-0625-4Published: 12 May 2021

  • eBook ISBN: 978-1-0716-0623-0Published: 11 May 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 5

  • Number of Pages: XIII, 392

  • Number of Illustrations: 1 b/w illustrations, 90 illustrations in colour

  • Topics: Genetics and Genomics

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