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  • © 2020

Characterization of Plant Viruses

Methods and Protocols

  • Includes comprehensive and complete practical manual containing protocols for identification, characterisation and detection of viruses infecting plants based on biological, physico-chemical, serological and nucleic acid properties of the virus

  • Provides all recent methods in virus identification including lateral flow device, loop-mediated isothermal amplification, rolling circle amplification, next generation sequencing approaches and microarray

  • Serves as a single source of information and methods of preparation of all reagents required for Plant Virology works

Part of the book series: Springer Protocols Handbooks (SPH)

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  • ISBN: 978-1-0716-0334-5
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Softcover Book USD 159.99
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Table of contents (50 protocols)

  1. Storage and Preservation of Plant Virus Cultures

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 125-131
  2. Purification of Plant Viruses

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 133-168
  3. Ultraviolet Absorption Spectra of Purified Virus Preparation

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 169-171
  4. Electron Microscopy and Utramicrotomy

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 173-183
  5. Determination of Coat Protein Molecular Weight of Viruses

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 185-190
  6. Isolation of Nucleic Acid from Purified Virus and Determination of Its Nature

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 191-198
  7. Agarose Gel Electrophoresis for Nucleic Acids

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 199-205
  8. In Vitro Expression of Viral Coat Protein in Prokaryotic System and Its Purification

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 207-213
  9. Production of Polyclonal Antiserum

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 215-221
  10. Production of Monoclonal Antibody

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 223-237
  11. Serological Tests

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 239-283
  12. Isolation of Total DNA from Plants

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 285-290
  13. Isolation of Total RNA from Plants

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 291-297
  14. Isolation of Double-Stranded (ds) RNA from Virus-Infected Plants

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 299-302
  15. Dot-Blot Hybridization Technique

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 303-321
  16. Polymerase Chain Reaction

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 323-345
  17. Real-Time Polymerase Chain Reaction

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 347-356
  18. DNA Microarray for Detection of Plant Viruses

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 357-367
  19. Loop-Mediated Isothermal Amplification (LAMP)

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 369-376
  20. Rolling Circle Amplification (RCA)

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 377-381

About this book

This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses onin vitro expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its scope, the book will be a valuable resource for every laboratory, student and teacher, and for everyone interested in plant virology, plant pathology, plant biology and molecular biology, offering them a practical manual on various aspects of plant viruses.

Keywords

  • Plant Virology
  • Virus Characterization
  • Purification Protocols
  • Serological Methods
  • Biotechnological Approaches

Authors and Affiliations

  • Division of Crop Protection, ICAR-Indian Institute of Spices Research, Kozhikode, India

    Alangar Ishwara Bhat

  • Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, India

    Govind Pratap Rao

About the authors

Dr Alangar Ishwara Bhat

Alangar Ishwara Bhat is currently working as a Principal Scientist at the ICAR-Indian Institute of Spices Research, Kozhikode, Kerala, India. Having obtained his PhD from the ICAR-Indian Agricultural Research Institute, New Delhi, he has 28 years of research experience in Plant Virology and has contributed significantly to the identification, characterization, diagnostics and management of viruses. Dr Bhat has been a visiting scientist at the University of Georgia, USA and at the Food and Environment Research Agency, UK. He has handled ten externally funded projects from the Government of India, authored 89 publications in reputed refereed journals, edited two books, and contributed 28 book chapters and three technical bulletins. He is the chief editor of the Journal of Spices and Aromatic Crops and a section editor of VirusDisease. His work has been recognized with several national awards.

Dr Govind Pratap Rao

Govind Pratap Rao is a Principal Scientist (Plant Pathology) at the Indian Agricultural Research Institute, New Delhi. He received his PhD in Plant Virology from Gorakhpur University in 1986 and pursued his postdoctoral research at the University of Urbana-Champaign, Illinois, USA (1994) and at Cedex, Montpellier, France (1998).  Dr Rao has 32 years of research experience, and has made significant contributions to the characterization of plant viruses and phytoplasmas. He has over 150 research articles, and 25 and authored and edited books to his credit.  His work has been recognized with several prestigious national and international awards. Dr Rao is also Secretary General of the Indian Virological Society, New Delhi and a member of several prestigious international scientific societies. In addition, he has travelled to more than 30 countries as a visiting scientist, expert, for invited talks, research training, panel discussions and conferences. 

Bibliographic Information

  • Book Title: Characterization of Plant Viruses

  • Book Subtitle: Methods and Protocols

  • Authors: Alangar Ishwara Bhat, Govind Pratap Rao

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-1-0716-0334-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0333-8

  • Softcover ISBN: 978-1-0716-0336-9

  • eBook ISBN: 978-1-0716-0334-5

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: XXI, 552

  • Number of Illustrations: 62 b/w illustrations, 99 illustrations in colour

  • Topics: Plant Pathology, Virology, Plant Genetics, Plant Physiology, Plant Development

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • ISBN: 978-1-0716-0334-5
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 159.99
Price excludes VAT (USA)
Hardcover Book USD 249.99
Price excludes VAT (USA)