Skip to main content
  • Book
  • © 2020

Characterization of Plant Viruses

Methods and Protocols

  • Includes comprehensive and complete practical manual containing protocols for identification, characterisation and detection of viruses infecting plants based on biological, physico-chemical, serological and nucleic acid properties of the virus

  • Provides all recent methods in virus identification including lateral flow device, loop-mediated isothermal amplification, rolling circle amplification, next generation sequencing approaches and microarray

  • Serves as a single source of information and methods of preparation of all reagents required for Plant Virology works

Part of the book series: Springer Protocols Handbooks (SPH)

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • ISBN: 978-1-0716-0334-5
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 159.99
Price excludes VAT (USA)
Hardcover Book USD 249.99
Price excludes VAT (USA)

This is a preview of subscription content, access via your institution.

Table of contents (50 protocols)

  1. Front Matter

    Pages i-xxi
  2. Glasshouse for Maintenance of Virus and Insect Culture

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 1-5
  3. Symptoms of Virus-Infected Plants

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 7-21
  4. Isolation and Diagnosis of Virus Through Indicator Hosts

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 23-27
  5. Host Range of Viruses

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 29-31
  6. Physico-chemical Properties of Virus in Crude Sap

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 33-38
  7. Mechanical Sap Transmission

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 39-43
  8. Transmission Through Grafting and Budding

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 45-56
  9. Transmission Through Dodder

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 57-60
  10. Virus Transmission Through Pollen

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 61-64
  11. Transmission Through Seeds

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 65-67
  12. Transmission of Viruses by Aphids

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 69-75
  13. Transmission of Viruses by Leafhoppers

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 77-82
  14. Transmission of Viruses by Whiteflies

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 83-87
  15. Transmission of Viruses by Thrips

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 89-93
  16. Transmission of Viruses Through Mealybugs

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 95-98
  17. Transmission of Viruses Through Beetles

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 99-103
  18. Transmission of Viruses Through Mites

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 105-109
  19. Transmission of Viruses Through Fungi

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 111-115
  20. Transmission of Viruses Through Nematodes

    • Alangar Ishwara Bhat, Govind Pratap Rao
    Pages 117-123

About this book

This book provides detailed information on methodologies used in biological, serological and nucleic acid based assays for the detection, diagnosis and management of plant viruses. The content is divided into six main parts, the first of which presents techniques used in the biological characterization and transmission of viruses, while Part II covers purification and techniques concerning the physico-chemical properties of viruses. In turn, Part III focuses onin vitro expression, production of polyclonal and monoclonal antibodies; and on various serological assays including precipitin tests, ELISA, blot immunoassays, immunosorbent electron microscopy and lateral flow immunoassays. Part IV addresses the isolation of DNA and RNA from plants and nucleic acid based assays such as dot-blot, polymerase chain reaction, real-time PCR, loop-mediated isothermal amplification, rolling circle amplification, recombinase polymerase amplification, and next-generation sequencing approaches. Part V discusses cloning, sequencing, sequence analysis and the production of infectious clones, while the last part (Part VI) provides biotechnological approaches for the management of viruses. Given its scope, the book will be a valuable resource for every laboratory, student and teacher, and for everyone interested in plant virology, plant pathology, plant biology and molecular biology, offering them a practical manual on various aspects of plant viruses.

Keywords

  • Plant Virology
  • Virus Characterization
  • Purification Protocols
  • Serological Methods
  • Biotechnological Approaches

Authors and Affiliations

  • Division of Crop Protection, ICAR-Indian Institute of Spices Research, Kozhikode, India

    Alangar Ishwara Bhat

  • Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, India

    Govind Pratap Rao

About the authors

Dr Alangar Ishwara Bhat

Alangar Ishwara Bhat is currently working as a Principal Scientist at the ICAR-Indian Institute of Spices Research, Kozhikode, Kerala, India. Having obtained his PhD from the ICAR-Indian Agricultural Research Institute, New Delhi, he has 28 years of research experience in Plant Virology and has contributed significantly to the identification, characterization, diagnostics and management of viruses. Dr Bhat has been a visiting scientist at the University of Georgia, USA and at the Food and Environment Research Agency, UK. He has handled ten externally funded projects from the Government of India, authored 89 publications in reputed refereed journals, edited two books, and contributed 28 book chapters and three technical bulletins. He is the chief editor of the Journal of Spices and Aromatic Crops and a section editor of VirusDisease. His work has been recognized with several national awards.

Dr Govind Pratap Rao

Govind Pratap Rao is a Principal Scientist (Plant Pathology) at the Indian Agricultural Research Institute, New Delhi. He received his PhD in Plant Virology from Gorakhpur University in 1986 and pursued his postdoctoral research at the University of Urbana-Champaign, Illinois, USA (1994) and at Cedex, Montpellier, France (1998).  Dr Rao has 32 years of research experience, and has made significant contributions to the characterization of plant viruses and phytoplasmas. He has over 150 research articles, and 25 and authored and edited books to his credit.  His work has been recognized with several prestigious national and international awards. Dr Rao is also Secretary General of the Indian Virological Society, New Delhi and a member of several prestigious international scientific societies. In addition, he has travelled to more than 30 countries as a visiting scientist, expert, for invited talks, research training, panel discussions and conferences. 

Bibliographic Information

  • Book Title: Characterization of Plant Viruses

  • Book Subtitle: Methods and Protocols

  • Authors: Alangar Ishwara Bhat, Govind Pratap Rao

  • Series Title: Springer Protocols Handbooks

  • DOI: https://doi.org/10.1007/978-1-0716-0334-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0333-8

  • Softcover ISBN: 978-1-0716-0336-9

  • eBook ISBN: 978-1-0716-0334-5

  • Series ISSN: 1949-2448

  • Series E-ISSN: 1949-2456

  • Edition Number: 1

  • Number of Pages: XXI, 552

  • Number of Illustrations: 62 b/w illustrations, 99 illustrations in colour

  • Topics: Plant Pathology, Virology, Plant Genetics, Plant Physiology, Plant Development

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • ISBN: 978-1-0716-0334-5
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 159.99
Price excludes VAT (USA)
Hardcover Book USD 249.99
Price excludes VAT (USA)