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Trypanosomatids

Methods and Protocols

  • Book
  • © 2020

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Editors:
  1. Paul A. M. Michels

    1. School of Biological Sciences, University of Edinburgh, Edinburgh, UK

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  2. Michael L. Ginger

    1. School of Applied Sciences, University of Huddersfield, Huddersfield, UK

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    You can also search for this editor in PubMed   Google Scholar

  3. Dan Zilberstein

    1. Faculty of Biology, Technion–Israel Institute of Technology, Haifa, Israel

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2116)

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About this book

This volume explores the latest methods used by researchers to study different trypanosomatid parasites. These methods cover numerous disciplines, from organismal biology to molecular mechanism. The chapters in this book cover topics such as high-throughput sequencing; next-generation analysis of trypanosomatid genome stability and instability; DNA repair in cell extracts; ribosome profiling; and the use of CRISPR/Cas9 technology for gene editing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Trypanosomatids: Methods and Protocols is a valuable resource for any researcher working with trypanosomatids and trypanosomatid-borne diseases. 

Chapters 14, 15, 16, 23, 24, 30, and 48 are open access under a CC BY 4.0 license.


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Table of contents (48 protocols)

  1. Front Matter

    Pages i-xxi
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  2. Isolation, Culturing, and Genetic Manipulation of Trypanosomatids

    1. Front Matter

      Pages 1-1
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    2. Field Isolation and Cultivation of Trypanosomatids from Insects

      • Julius Lukeš, Jan Votýpka
      Pages 3-21

    3. Culturing and Transfection of Pleomorphic Trypanosoma brucei

      • Sabine Bachmaier, Theresa Thanner, Michael Boshart
      Pages 23-38

    4. In Vitro Culture for Differentiation Simulation of Leishmania spp.

      • Dan Zilberstein
      Pages 39-47

    5. Tsetse Fly Transmission Studies of African Trypanosomes

      • Lori Peacock, Wendy Gibson
      Pages 49-67

    6. Infecting Triatomines with Trypanosomes

      • Alessandra A. Guarneri
      Pages 69-79

  3. Trypanosomatid Genome-Wide Analyses

    1. Front Matter

      Pages 81-81
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    2. High-Throughput Sequencing for Trypanosome Transcriptome Characterization

      • Julius Mulindwa, Kevin Leiss, Christine Clayton
      Pages 83-98

    3. Polysome Profiling and Metabolic Labeling Methods to Measure Translation in Trypanosoma brucei

      • Kathrin Bajak, Christine Clayton
      Pages 99-108

    4. Immunoprecipitation for the Analysis of Macromolecular Complexes in Trypanosoma cruzi

      • Bruno Accioly Alves Romagnoli, Samuel Goldenberg, Lysangela Ronalte Alves
      Pages 109-116

    5. Analysis of the In Vivo Translation Process in Trypanosoma cruzi Using Ribosome Profiling

      • Saloe Bispo Poubel, Fabiola Barbieri Holetz, Bruno Accioly Alves Romagnoli, Samuel Goldenberg, Lysangela Ronalte Alves
      Pages 117-123

    6. Proteome-Wide Quantitative Phosphoproteomic Analysis of Trypanosoma brucei Insect and Mammalian Life Cycle Stages

      • Corinna Benz, Michael D. Urbaniak
      Pages 125-137

    7. Genome-Wide Proteomics and Phosphoproteomics Analysis of Trypanosoma cruzi During Differentiation

      • Michel Batista, Juliana Carolina Amorim, Aline Castro Rodrigues Lucena, Fernanda Grande Kugeratski, Carla Vanessa de Paula Lima, Fabricio Klerynton Marchini
      Pages 139-159

    8. Genome-Wide Proteomics and Phosphoproteomics Analysis of Leishmania spp. During Differentiation

      • Harsh Pawar, Gajanan Sathe, Milind S. Patole
      Pages 161-176

    9. CRISPR/Cas9 Technology Applied to the Study of Proteins Involved in Calcium Signaling in Trypanosoma cruzi

      • Noelia Lander, Miguel A. Chiurillo, Roberto Docampo
      Pages 177-197

    10. Application of CRISPR/Cas9-Mediated Genome Editing in Leishmania

      • Wen-Wei Zhang, Patrick Lypaczewski, Greg Matlashewski
      Pages 199-224Open Access

    11. Next-Generation Analysis of Trypanosomatid Genome Stability and Instability

      • Emma M. Briggs, Catarina A. Marques, Joao Reis-Cunha, Jennifer Black, Samantha Campbell, Jeziel Damasceno et al.
      Pages 225-262Open Access

  4. Molecular Biology and Genetics

    1. Front Matter

      Pages 263-263
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    2. Transcription Factor Analysis in Trypanosomatids

      • Arthur Günzl, Ankita Srivastava, Ujwala Gosavi
      Pages 265-284Open Access
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Editors and Affiliations

  • School of Biological Sciences, University of Edinburgh, Edinburgh, UK

    Paul A. M. Michels

  • School of Applied Sciences, University of Huddersfield, Huddersfield, UK

    Michael L. Ginger

  • Faculty of Biology, Technion–Israel Institute of Technology, Haifa, Israel

    Dan Zilberstein

Bibliographic Information

  • Book Title: Trypanosomatids

  • Book Subtitle: Methods and Protocols

  • Editors: Paul A. M. Michels, Michael L. Ginger, Dan Zilberstein

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-0294-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-0716-0293-5Published: 28 March 2020

  • Softcover ISBN: 978-1-0716-0296-6Published: 28 March 2021

  • eBook ISBN: 978-1-0716-0294-2Published: 27 March 2020

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XXI, 823

  • Number of Illustrations: 50 b/w illustrations, 101 illustrations in colour

  • Topics: Infectious Diseases, Human Genetics

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