Plant Cryopreservation: A Practical Guide

  • Barbara M. Reed

Table of contents

  1. Front Matter
    Pages i-xxix
  2. Introduction to Cryopreservation

    1. Barbara M. Reed
      Pages 3-13
    2. Erica E. Benson
      Pages 15-32
    3. Florent Engelmann, Maria-Teresa Gonzalez Arnao, Yongjie Wu, Roosevelt Escobar
      Pages 59-75
    4. Barbara M. Reed, Esther Uchendu
      Pages 77-92
  3. Cryopreservation Protocols for Working Laboratories

    1. John G. Day, Keith Harding
      Pages 95-116
    2. Valerie C. Pence
      Pages 117-140
    3. Elke Heine-Dobbernack, Heiko Kiesecker, Heinz Martin Schumacher
      Pages 141-176
    4. Maurizio Lambardi, E. Aylin Ozudogru, Carla Benelli
      Pages 177-210
    5. M. N. Normah, A. M. Makeen
      Pages 211-240
    6. Bart Panis
      Pages 241-280
    7. E. R. Joachim Keller, Angelika Senula, Anja Kaczmarczyk
      Pages 281-332
    8. Barbara M. Reed
      Pages 333-364
    9. Hely Häggman, Mari Rusanen, Soile Jokipii
      Pages 365-386
    10. Yanhua Zhao, Yongjie Wu, Yongjian Chang, Barbara M. Reed
      Pages 387-420
    11. L. E. Towill, D. D. Ellis
      Pages 421-442
    12. S. Ganeshan, P. E. Rajasekharan, S. Shashikumar, William Decruze
      Pages 443-464
    13. Christina Walters, James Wesley-Smith, Jennifer Crane, Lisa M. Hill, Pawel Chmielarz, Norman W. Pammenter et al.
      Pages 465-484
    14. Hugh W. Pritchard, Jayanthi Nadarajan
      Pages 485-501
  4. Back Matter
    Pages 503-513

About this book


Plant Cryopreservation: A Practical Guide  is a unique resource for plant scientists in many fields. This compilation provides over 100 ready-to-use protocols for plant types from algae and bryophytes to a range of flowering plants. It includes techniques for diverse plant parts such as dormant buds, pollen, and apical meristems, and for cell types such as suspension and callus cultures. Five introductory chapters describe theoretical principles, practical aspects of long-term cryopreserved storage, and details of the main cryopreservation techniques. The remaining 14 chapters are separated by plant type. Each chapter briefly reviews the literature and includes ready-to-use protocols designed for easy transfer into the lab and adaptation to new species.


Bryophyte Embryo Fruit Seed algae crops germplasm horticulture plant biotechnology plant cryopreservation

Editors and affiliations

  • Barbara M. Reed
    • 1
  1. 1.USDA-ARS National Clonal Germplasm RepositoryCorvallisUSA

Bibliographic information