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Electron Tomography

Methods for Three-Dimensional Visualization of Structures in the Cell

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  • © 2006
  • Latest edition

Overview

  • Presents the mathematical background and working methods for three-dimensional reconstruction from projections

  • Takes the reader from biological specimen preparation to three-dimensional images of the cell and its components

  • Revised and updated extensively from the first edition published in 1992

  • The definitive work in the field written by leading international experts

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About this book

Electron tomography has become a standard technique with applications in cell biology, structural biology, and materials science. This definitive work provides a comprehensive treatment of the mathematical background and working methods of three-dimensional reconstruction from tilt series, with special emphasis on the problems presented by limitations of data collection in the transmission electron microscope. In addition to chapters that are applicable to 3D reconstruction in all fields of science, such as radiological imaging in medicine and electron tomography in materials science, Electron Tomography also focuses on specimen preparation and imaging unique to biological electron microscopy.

This extensively revised second edition updates key contributions on the mathematics of 3D reconstruction, and includes new topics such as automated tomography, frozen sectioning of cells, and the interpretation of density maps through methods of fitting, docking, denoising, and segmentation. Each chapter is a self-contained treatise by a world expert in the author's field of research, resulting in an indispensable resource and companion for laboratories that practice electron tomography or seek to implement electron tomography as a tool for visualization of cells and cell components.

 Key Features

  • Presents the mathematical background and working methods for three-dimensional reconstruction from projections
  • Takes the reader from biological specimen preparation to three-dimensional images of the cell and its components
  • Revised and updated extensively from the first edition published in 1992
  • The definitive work in the field written by leading international experts

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Keywords

Table of contents (16 chapters)

Editors and Affiliations

  • Wadsworth Center and State University of New York, Albany, USA

    Joachim Frank

Bibliographic Information

  • Book Title: Electron Tomography

  • Book Subtitle: Methods for Three-Dimensional Visualization of Structures in the Cell

  • Editors: Joachim Frank

  • DOI: https://doi.org/10.1007/978-0-387-69008-7

  • Publisher: Springer New York, NY

  • eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)

  • Copyright Information: Springer-Verlag New York 2006

  • Hardcover ISBN: 978-0-387-31234-7Published: 18 December 2006

  • Softcover ISBN: 978-1-4419-2172-7Published: 25 November 2010

  • eBook ISBN: 978-0-387-69008-7Published: 05 March 2008

  • Edition Number: 2

  • Number of Pages: XIV, 456

  • Number of Illustrations: 123 b/w illustrations

  • Topics: Biological Microscopy, Characterization and Evaluation of Materials, Cell Biology

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