© 2006

Handbook Of Biological Confocal Microscopy

  • James B. Pawley
  • Includes detailed descriptions and in-depth comparisons of parts of the microscope itself

  • Covers dyes and techniques of 3D specimen prep, as well as digital data acquisition

  • Addresses the fundamental limitations and practical complexities of quantitative confocal fluorescence imaging

  • This is the revised and expanded Third Edition of a classic text


Table of contents

  1. Ping-Chin Cheng
    Pages 414-441
  2. Colin J. R. Sheppard, Xiaosong Gan, Min Gu, Maitreyee Roy
    Pages 442-452
  3. Timothy J. Holmes, David Biggs, Asad Abu-Tarif
    Pages 468-487
  4. Mark B. Cannell, Angus McMorland, Christian Soeller
    Pages 488-500
  5. Peter Delaney, Martin Harris
    Pages 501-515
  6. H. C. Gerritsen, A. Draaijer, D. J. van den Heuvel, A. V. Agronskaia
    Pages 516-534
  7. Winfried Denk, David W. Piston, Watt W. Webb
    Pages 535-549
  8. Jörg Bewersdorf, Alexander Egner, Stefan W. Hell
    Pages 550-560
  9. Jörg Bewersdorf, Alexander Egner, Stefan W. Hell
    Pages 561-570
  10. Stefan W. Hell, Katrin I. Willig, Marcus Dyba, Stefan Jakobs, Lars Kastrup, Volker Westphal
    Pages 571-579
  11. Guy Cox
    Pages 580-594
  12. X. Sunney Xie, Ji-Xin Cheng, Eric Potma
    Pages 595-606
  13. J. Michael Tyszka, Seth W. Ruffins, Jamey P. Weichert, Michael J. Paulus, Scott E. Fraser
    Pages 607-626
  14. Alan R. Hibbs, Glen MacDonald, Karl Garsha
    Pages 650-671
  15. Jan Huisken, Jim Swoger, Steffen Lindek, Ernst H. K. Stelzer
    Pages 672-679
  16. Karsten König
    Pages 680-689
  17. Alberto Diaspro, Giuseppe Chirico, Cesare Usai, Paola Ramoino, Jurek Dobrucki
    Pages 690-702

About this book


Once the second edition was safely off to the printer, the 110 larger world of micro-CT and micro-MRI and the smaller world authors breathed a sigh of relief and relaxed, secure in the belief revealed by the scanning and transmission electron microscopes. that they would “never have to do that again. ” That lasted for 10 To round out the story we even have a chapter on what PowerPoint years. When we ?nally awoke, it seemed that a lot had happened. does to the results, and the annotated bibliography has been In particular, people were trying to use the Handbook as a text- updated and extended. book even though it lacked the practical chapters needed. There As with the previous editions, the editor enjoyed a tremendous had been tremendous progress in lasers and ?ber-optics and in our amount of good will and cooperation from the 124 authors understanding of the mechanisms underlying photobleaching and involved. Both I, and the light microscopy community in general, phototoxicity. It was time for a new book. I contacted “the usual owe them all a great debt of gratitude. On a more personal note, I suspects” and almost all agreed as long as the deadline was still a would like to thank Kathy Lyons and her associates at Springer for year away.


Biological Confocal Microscopy Pawley chemistry databases electron microscopy fluorescence preparation

Editors and affiliations

  • James B. Pawley
    • 1
  1. 1.Department of ZoologyUniversity of WisconsinMadisonUSA

Bibliographic information