Overview
- Editors:
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James B. Pawley
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Department of Zoology, University of Wisconsin, Madison, USA
Includes detailed descriptions and in-depth comparisons of parts of the microscope itself
Covers dyes and techniques of 3D specimen prep, as well as digital data acquisition
Addresses the fundamental limitations and practical complexities of quantitative confocal fluorescence imaging
This is the revised and expanded Third Edition of a classic text
Includes supplementary material: sn.pub/extras
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Table of contents (52 chapters)
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- Alexander Egner, Stefan W. Hell
Pages 404-413
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- Colin J. R. Sheppard, Xiaosong Gan, Min Gu, Maitreyee Roy
Pages 442-452
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- Timothy J. Holmes, David Biggs, Asad Abu-Tarif
Pages 468-487
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- Mark B. Cannell, Angus McMorland, Christian Soeller
Pages 488-500
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- Peter Delaney, Martin Harris
Pages 501-515
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- H. C. Gerritsen, A. Draaijer, D. J. van den Heuvel, A. V. Agronskaia
Pages 516-534
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- Winfried Denk, David W. Piston, Watt W. Webb
Pages 535-549
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- Jörg Bewersdorf, Alexander Egner, Stefan W. Hell
Pages 550-560
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- Jörg Bewersdorf, Alexander Egner, Stefan W. Hell
Pages 561-570
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- Stefan W. Hell, Katrin I. Willig, Marcus Dyba, Stefan Jakobs, Lars Kastrup, Volker Westphal
Pages 571-579
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- X. Sunney Xie, Ji-Xin Cheng, Eric Potma
Pages 595-606
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- J. Michael Tyszka, Seth W. Ruffins, Jamey P. Weichert, Michael J. Paulus, Scott E. Fraser
Pages 607-626
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- Victoria Centonze, James B. Pawley
Pages 627-649
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- Alan R. Hibbs, Glen MacDonald, Karl Garsha
Pages 650-671
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- Jan Huisken, Jim Swoger, Steffen Lindek, Ernst H. K. Stelzer
Pages 672-679
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- Alberto Diaspro, Giuseppe Chirico, Cesare Usai, Paola Ramoino, Jurek Dobrucki
Pages 690-702
About this book
Once the second edition was safely off to the printer, the 110 larger world of micro-CT and micro-MRI and the smaller world authors breathed a sigh of relief and relaxed, secure in the belief revealed by the scanning and transmission electron microscopes. that they would “never have to do that again. ” That lasted for 10 To round out the story we even have a chapter on what PowerPoint years. When we ?nally awoke, it seemed that a lot had happened. does to the results, and the annotated bibliography has been In particular, people were trying to use the Handbook as a text- updated and extended. book even though it lacked the practical chapters needed. There As with the previous editions, the editor enjoyed a tremendous had been tremendous progress in lasers and ?ber-optics and in our amount of good will and cooperation from the 124 authors understanding of the mechanisms underlying photobleaching and involved. Both I, and the light microscopy community in general, phototoxicity. It was time for a new book. I contacted “the usual owe them all a great debt of gratitude. On a more personal note, I suspects” and almost all agreed as long as the deadline was still a would like to thank Kathy Lyons and her associates at Springer for year away.
Editors and Affiliations
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Department of Zoology, University of Wisconsin, Madison, USA
James B. Pawley
