© 2006

Handbook Of Biological Confocal Microscopy

  • James B. Pawley
  • Includes detailed descriptions and in-depth comparisons of parts of the microscope itself

  • Covers dyes and techniques of 3D specimen prep, as well as digital data acquisition

  • Addresses the fundamental limitations and practical complexities of quantitative confocal fluorescence imaging

  • This is the revised and expanded Third Edition of a classic text


Table of contents

  1. Front Matter
    Pages i-xxviii
  2. James B. Pawley
    Pages 20-42
  3. Jens Rietdorf, Ernst H. K. Stelzer
    Pages 43-58
  4. Enrico Gratton, Martin J. vandeVen
    Pages 80-125
  5. Andreas Nolte, James B. Pawley, Lutz Höring
    Pages 126-144
  6. H. Ernst Keller
    Pages 145-161
  7. Ping-Chin Cheng
    Pages 162-206
  8. Derek Toomre, James B. Pawley
    Pages 221-238
  9. Jonathan Art
    Pages 251-264
  10. Rainer Heintzmann
    Pages 265-279
  11. Badrinath Roysam, Gang Lin, Muhammad-Amri Abdul-Karim, Omar Al-Kofahi, Khalid Al-Kofahi, William Shain et al.
    Pages 316-337
  12. Roger Y. Tsien, Lauren Ernst, Alan Waggoner
    Pages 338-352
  13. Robert Bacallao, Sadaf Sohrab, Carrie Phillips
    Pages 368-380
  14. Michael E. Dailey, Erik Manders, David R. Soll, Mark Terasaki
    Pages 381-403

About this book


Once the second edition was safely off to the printer, the 110 larger world of micro-CT and micro-MRI and the smaller world authors breathed a sigh of relief and relaxed, secure in the belief revealed by the scanning and transmission electron microscopes. that they would “never have to do that again. ” That lasted for 10 To round out the story we even have a chapter on what PowerPoint years. When we ?nally awoke, it seemed that a lot had happened. does to the results, and the annotated bibliography has been In particular, people were trying to use the Handbook as a text- updated and extended. book even though it lacked the practical chapters needed. There As with the previous editions, the editor enjoyed a tremendous had been tremendous progress in lasers and ?ber-optics and in our amount of good will and cooperation from the 124 authors understanding of the mechanisms underlying photobleaching and involved. Both I, and the light microscopy community in general, phototoxicity. It was time for a new book. I contacted “the usual owe them all a great debt of gratitude. On a more personal note, I suspects” and almost all agreed as long as the deadline was still a would like to thank Kathy Lyons and her associates at Springer for year away.


Biological Confocal Microscopy Pawley chemistry databases electron microscopy fluorescence preparation

Editors and affiliations

  • James B. Pawley
    • 1
  1. 1.Department of ZoologyUniversity of WisconsinMadisonUSA

Bibliographic information