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Confocal Microscopy for Biologists

  • Alan R. Hibbs

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Alan R. Hibbs
    Pages 1-29
  3. Alan R. Hibbs
    Pages 31-63
  4. Alan R. Hibbs
    Pages 65-99
  5. Alan R. Hibbs
    Pages 101-144
  6. Alan R. Hibbs
    Pages 145-162
  7. Alan R. Hibbs
    Pages 163-176
  8. Alan R. Hibbs
    Pages 177-185
  9. Alan R. Hibbs
    Pages 187-200
  10. Alan R. Hibbs
    Pages 201-238
  11. Alan R. Hibbs
    Pages 239-257
  12. Alan R. Hibbs
    Pages 259-277
  13. Alan R. Hibbs
    Pages 279-323
  14. Alan R. Hibbs
    Pages 325-333
  15. Alan R. Hibbs
    Pages 335-346
  16. Alan R. Hibbs
    Pages 347-353
  17. Back Matter
    Pages 355-467

About this book

Introduction

There has been a great upsurge in interest in light microscopy in recent years due to the advent of a number of significant advances in microscopy, one of the most important of which is confocal microscopy. Confocal microscopy has now become an important research tool, with a large number of new fluorescent dyes becoming available in the past few years, for probing your pet structure or molecule within fixed or living cell or tissue sampies. Many of the people interested in using confocal microscopy to further their research do not have a background in microscopy or even cell biology and so not only do they find considerable difficulty in obtaining satisfactory results with a confocal microscope, but they may be mislead by how data is being presented. This book is intended to teach you the basic concepts ofmicroscopy, fluorescence, digital imaging and the principles of confocal microscopy so that you may take full advantage ofthe excellent confocal microscopes now available. This book is also an excellent reference source for information related to confocal microscopy for both beginners and the more advanced users. For example, do you need to know the optimal pinhole size for a 63x 1. 4 NA lens? Do you need to know the fluorescence emission spectrum of Alexa 568? Access to the wealth of practical information in this book is made easier by using both the detailed index and the extensive glossary.

Keywords

cell imaging techniques life sciences microscopy

Authors and affiliations

  • Alan R. Hibbs
    • 1
  1. 1.BIOCONMelbourneAustralia

Bibliographic information