Basic Methods for the Biochemical Lab

  • Martin¬†Holtzhauer

Part of the Springer Lab Manuals book series (SLM)

Table of contents

  1. Front Matter
    Pages I-XVIII
  2. Pages 23-82
  3. Pages 83-128
  4. Pages 129-160
  5. Pages 161-180
  6. Pages 181-189
  7. Pages 191-207
  8. Pages 209-232
  9. Back Matter
    Pages 247-251

About this book

Introduction

Basic Methods for the Biochemical Lab elucidates proven lab procedures and practical hints for research in analytical and preparative biochemistry, as well as summarizing key data in numerous tables. To further emphasize the practical aspects and to minimize the text, theoretical introductions into the methods are mostly omitted.

The chapters cover: quantitative methods (proteins, nucleic acids, phospholipids and carbohydrates), electrophoresis (several polyacrylamide and agarose systems, 2D-PAGE, detection methods and affinity electrophoresis), chromatographic protocols (thin-layer chromatography, GPC, IEC, affinity chromatography, HPLC), immunochemical protocols (hapten-carrier and enzyme conjugation, immunization and antibody purification, immune affinity chromatography, ELISA), centrifugation (differential and density gradient centrifugation for cells and cellular fractions), radioactivity (labeling and counting), buffers (buffer properties and compositions).

In additional chapters, tables compile a broad array of useful information, e.g. SI units conversion factor, detergent, protein and nucleotide data, and the basic principles of statistics and enzyme and receptor kinetics are reviewed.

This first English-language edition of a successful German-language manual will be a valuable resource for students and experts in biochemistry, biotechnology and biomedical laboratories.

Keywords

Analytical Biochemistry Lab Manual Laboratory Nucleotide Protocols biochemistry enzymes proteins

Authors and affiliations

  • Martin¬†Holtzhauer
    • 1
  1. 1.Human GmbH Branch IMTECBerlinGermany

Bibliographic information

  • DOI https://doi.org/10.1007/3-540-32786-X
  • Copyright Information Springer-Verlag Berlin Heidelberg 2006
  • Publisher Name Springer, Berlin, Heidelberg
  • eBook Packages Biomedical and Life Sciences
  • Print ISBN 978-3-540-32785-1
  • Online ISBN 978-3-540-32786-8
  • About this book