Methods in Gut Microbial Ecology for Ruminants

  • Harinder P.S. Makkar
  • Christopher S. McSweeney

Table of contents

  1. Front Matter
    Pages i-xi
  2. Designing **in vivo microbial ecology studies

  3. Classical methods for isolation, enumeration, cultivation and functional assays of rumen microbes

    1. Christopher S. McSweeney, Stuart E. Denman, Roderick I. Mackie
      Pages 23-37
    2. Athol V. Klieve
      Pages 39-46
    3. Keith N. Joblin
      Pages 47-53
    4. Michael K. Theodorou, Jayne Brookman, Anthony P.J. Trinci
      Pages 55-66
    5. Burk A. Dehority
      Pages 67-78
  4. PCR-based methods for analysis of populations and gene expression

    1. Zhongtang Yu, Robert J. Forster
      Pages 81-104
    2. Stuart E. Denman, Christopher S. McSweeney
      Pages 105-115
  5. Molecular fingerprinting techniques for genotypic analysis of pure cultures and microbial communities

    1. Svetlana A. Kocherginskaya, Isaac K.O. Cann, Roderick I. Mackie
      Pages 119-128
    2. Athol V. Klieve, Rosalind A. Gilbert
      Pages 129-137
    3. Jayne L. Brookman, Matthew J. Nicholson
      Pages 139-150
    4. Stuart E. Denman, Makoto Mitsumori, Christopher S. Mcsweeney
      Pages 151-159
  6. DNA clone libraries of microbial communities

    1. André-Denis G. Wright, Kiyoshi Tajima, Rustam I. Aminov
      Pages 163-174
  7. Use of small subunit ribosomal RNA directed oligonucleotide probes for microbial population studies

  8. Genomic analysis of microbial ecosystems

    1. Mark Morrison, Sarah E. Adams, Karen E. Nelson, Graeme T. Attwood
      Pages 209-220
  9. Back Matter
    Pages 221-225

About this book


Asaresultofvarioushumanactivities,suchasincreaseinhumanpopulation,decrease in arable land due to soil degradation, urbanization, industrialization and associated increase in the demand for livestock products, dramatic changes are occurring in the global ruminant livestock sector. These changes includeshift inthesize of regional livestock populations and in the types of management and feeding systems under which ruminant livestock are held, and increased demand of a wider range of quality attributes from animal agriculture, not just of the products themselves but also of the methods used in their production. The livestock sector will need to respond to newchallengesofincreasinglivestockproductivitywhileprotectingenvironmentand human health and conservingbiodiversity and natural resources. The micro-organisms in the digestive tracts of ruminant livestock have a profound in?uence on the conversion offeedinto end products, which can impact on the- imal and theenvironment. As the livestock sector grows particularly in developing countries, there will be an increasing need to understand these processes for b- ter management and use ofbothfeed and other natural resources that underpinthe development of sustainable feeding systems.


Biome Ecology Expression FISH Nucleotide PCR gene expression genes hybridization microorganisms rDNA ribosomal RNA

Editors and affiliations

  • Harinder P.S. Makkar
    • 1
  • Christopher S. McSweeney
    • 2
  1. 1.Animal Production and Health Section Joint FAO/IAEA Division of Nuclear Techniques in Food and AgricultureInternational Atomic Energy AgencyViennaAustria
  2. 2.CSIRO Livestock IndustriesQueensland Bioscience PrecinctSt LuciaAustralia

Bibliographic information