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From Cells to Proteins: Imaging Nature across Dimensions

Proceedings of the NATO Advanced Study Institute on From Cells to Proteins: Imaging Nature across Dimensions Pisa, Italy 12–23 September 2004

  • Valtere Evangelista
  • Laura Barsanti
  • Vincenzo Passarelli
  • Paolo Gualtieri

Part of the NATO Security through Science Series book series

Table of contents

  1. Front Matter
    Pages i-xii
  2. John M. Girkin
    Pages 1-22
  3. Franco Verni, Simone Gabrielli
    Pages 23-35
  4. Giovanna Rosati, Simone Gabrielli
    Pages 37-52
  5. Massimo Sandal, Giampaolo Zuccheri, Bruno Samorì
    Pages 111-137
  6. Ille C. Gebeshuber, Richard A. P. Smith, Hannspeter Winter, Friedrich Aumayr
    Pages 139-165
  7. Tom W. Ford
    Pages 167-185
  8. Alberto Diaspro, Paolo Bianchini, Valentina Caorsi, Davide Mazza, Mattia Pesce, Ilaria Testa et al.
    Pages 187-207
  9. Thomas M. Jovin, Diane S. Lidke, Elizabeth A. Jares-Erijman
    Pages 209-216
  10. Donna J. Arndt-Jovin, Thomas M. Jovin, Diane S. Lidke
    Pages 217-225
  11. Valtere Evangelista, Laura Barsanti, Anna Maria Frassanito, Vincenzo Passarelli, Paolo Gualtieri
    Pages 323-333
  12. Primo Coltelli, Laura Barsanti, Valtere Evangelista, Anna Maria Frassanito, Paolo Gualtieri
    Pages 335-348
  13. M. Bertero, P. Boccacci
    Pages 349-370
  14. Giuseppe Vicidomini
    Pages 371-393
  15. Francesca Sbrana, Laura Barsanti, Vincenzo Passarelli, Paolo Gualtieri
    Pages 395-403
  16. Evgeniy V. Dubrovin, Inessa A. Kirik, Michael M. Babykin, Igor V. Yaminsky
    Pages 405-414
  17. Tatyana V. Parshikova
    Pages 415-421
  18. E. Korchemskaya, D. Stepanchikov, S. Bugaychuk, N. Burykin
    Pages 431-450
  19. Back Matter
    Pages 471-479

About these proceedings

Introduction

How deep we can see inside Nature's smallest secrets? Will it be possible some day in the near future to investigate living structures at atomic level? This area of study is very interdisciplinary, since it applies the principles and the techniques of biology, physics, chemistry, mathematics, and engineering to elucidate the structures of biological macromolecules, of supramolecular structures, organelles, and cells. This book offers updated information on how much information we are able to obtain in the exploration of the inner details of biological specimens in their native structure and composition.

The book deals with the implementation of laser beam and stage scanning systems incorporating confocal optics or multiphoton microscopy; the advent of new electro-optical detectors with great sensitivity, linearity, and dynamic range; the possibility of 2D fast image enhancement, reconstruction, restoration, analysis and 3D display, and the application of luminescence techniques (FLIMT, FRET combined with the use of quantum dots), which gives the possibility to investigate the chemical and molecular spatio-temporal organization of life processes; Electron Microscopy and Scanning Force Microscopy (SFM), are also presented, which has opened completely new perspectives for analyzing the surface topography of biological matter in its aqueous environment at a resolution comparable to that achieved by EM.

Keywords

Monitoring NMR Organelle Vivo automation biology display electron microscopy image analysis linearity

Editors and affiliations

  • Valtere Evangelista
    • 1
  • Laura Barsanti
    • 1
  • Vincenzo Passarelli
    • 1
  • Paolo Gualtieri
    • 1
  1. 1.CNR Institute of BiophysicsPisaItaly

Bibliographic information