© 2005

Liquid Culture Systems for in vitro Plant Propagation

  • Anne Kathrine Hvoslef-Eide
  • Walter Preil


  • Unique bookdetailing liquid-media systems for plant tissue culture

  • Contains contributions from world authorities

  • First-hand practical applications are detailed from crops

  • Uses a wide range of techniques


Table of contents

  1. Front Matter
    Pages i-xviii
  2. Bioreactors

  3. Temporary Immersion Systems

    1. Nilca Albany, Elio Jiménez González, Jorge Vilchez, Leyanis García, Manuel de Feria, Naivy Pérez et al.
      Pages 213-224
    2. Rafael Gómez Kosky, J. Vilchez Perozo, N. Albany Valero, D. Agramonte Peñalver
      Pages 225-229
    3. C. Damiano, S.R. La Starza, S. Monticelli, A. Gentile, E. Caboni, A. Frattarelli
      Pages 243-251
    4. Katerina Grigoriadou, Miltiadis Vasilakakis, Theofilos Tzoulis, Eleftherios P. Eleftheriou
      Pages 263-274

About this book


High-efficiency micropropagation, with relatively low labour costs, has been demonstrated in this unique book detailing liquid media systems for plant tissue culture. World authorities (e.g. von Arnold, Curtis, Takayama, Ziv) contribute seminal papers together with papers from researchers across Europe that are members of the EU COST Action 843 "Advanced micropropagation systems". First-hand practical applications are detailed for crops – including ornamentals and trees – using a wide range of techniques, from thin-film temporary immersion systems to more traditional aerated bioreactors with many types of explant – shoots to somatic embryos. The accounts are realistic, balanced and provide a contemporary account of this important aspect of mass propagation.

This book is essential reading for all those in commercial micropropagation labs, as well as researchers worldwide who are keen to improve propagation techniques and lower economic costs of production. Undergraduate and postgraduate students in the applied plant sciences and horticulture will find the book an enlightened treatise.


Embryo Hydra Regeneration Sowing seed

Editors and affiliations

  • Anne Kathrine Hvoslef-Eide
    • 1
  • Walter Preil
    • 2
  1. 1.Department of Plant and Environmental ScienceAgricultural University of NorwayÅsNorway
  2. 2.Formerly Federal Centre for Breeding Research on Cultivated PlantsInstitute for Ornamental Plant BreedingAhrensburgGermany

Bibliographic information


From the reviews:

"This book describes very well recent developments in liquid culture system for in vitro plant propagation. It will be an excellent source of information to the researchers for refining methodologies on large-scale plant propagation and secondary metabolite production. … This book is highly recommended to the researchers and students, and libraries." (S. Mohan Jain, Plant Cell, Tissue and Organ Culture, Vol. 84, 2006)