Photoautotrophic (sugar-free medium) Micropropagation as a New Micropropagation and Transplant Production System

1. Front Matter

   Pages i-xiii

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2. Introduction

   • T. Kozai

   Pages 1-5

3. Units and Terminology Use for the Studies of Photoautotrophic Micropropagation

   • T. Kozai, C. Kubota

   Pages 7-18

4. Concepts, Definitions, Ventilation Methods, Advantages and Disadvantages

   • T. Kozai, C. Kubota

   Pages 19-30

5. In Vitro Aerial Environments and Their Effects on Growth and Development of Plants

   • T. Kozai, C. Kubota

   Pages 31-52

6. In Vitro Root Zone Environments and Their Effects on Growth and Development of Plants

   • T. Kozai, C. Kubota

   Pages 53-60

7. Physiological and Anatomical Characteristics of in Vitro Photoautotrophic Plants

   • F. Afreen

   Pages 61-90

8. Photoautotrophic Plant Conversion in the Process of Somatic Embryogenesis

   • F. Afreen, S.M.A. Zobayed

   Pages 91-122

9. Photoautotrophic Micro-Propagation of Woody Species

   • Q. T. Nguyen, T. Kozai

   Pages 123-146

10. Ventilation in Micropropagation

    • S. M. A. Zobayed

    Pages 147-186

11. A Commercialized Photoautotrophic Micropropagation System Using Large Vessels with Forced Ventilation

    • Y. Xiao, T. Kozai

    Pages 187-204

12. Low Temperature Storage of Plants Under Dim Light

    • C. Kubota

    Pages 205-212

13. Modelling and Simulation for Production Planning in Photo Autotrophic Micropropagation

    • C. Kubota

    Pages 213-224

14. Modelling and Simulation in Photoautotrophic Micropropagation

    • G. Niu

    Pages 225-236

15. Frequently Asked Questions

    • C. Kubota, T. Kozai

    Pages 237-242

16. Plant Species Successfully Micropropagated Photoautotrophically

    • C. Kubota, F. Afreen, S. M. A. Zobayed

    Pages 243-266

17. Reconsideration of Conventional Micropropagation Systems

    • T. Kozai

    Pages 267-274

18. Closed Systems with Lamps for High Quality Transplant Production at Low Costs Using Minimum Resources

    • T. Kozai

    Pages 275-311

19. Concluding Remarks

    • S. M. A. Zobayed, F. Afreen

    Pages 313-314

20. Back Matter

    Pages 315-316

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This book provides two basic concepts on plant propagation and value-added transplant production in a closed structure with artificial lighting: 1) photoautotrophic (sugar-free medium, photosynthetic or inorganic nutrition) micropropagation systems, and 2) closed transplant production systems with minimum resource consumption and environmental pollution. This book also describes the methodology, technology and practical techniques employed in both systems, which have been commercialized recently in some Asian countries such as China and Japan. We often use a closed structure such as a tissue culture vessel, a culture room, a growth chamber, a plant factory with lamps, and a greenhouse to propagate plants and produce transplants. Main reasons why we use such a closed structure is: 1) higher controllability of the environment for desired plant growth, 2) easier protection of plants from damage by harsh physical environment, pathogens, insects, animals, etc, 3) easier reduction in resource consumption for environmental control and protection, and 4) higher quality and productivity of plants at a lower cost, compared with the plant propagation and transplant production under rain, wind and sunlight shelters and in the open fields. Thus, there should be some knowledge, discipline, methodology, technology and problems to be solved on plant propagation and transplant production common to those closed structures, regardless of the types and sizes of the closed structure.

• Embryo
• Hydra
• Transpiration
• biotechnology
• plant development

• Chiba University, Chiba-shi, Japan

  T. Kozai

• University of Guelph, Canada

  F. Afreen, S.M.A. Zobayed

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