Nature's Versatile Engine:

1. Front Matter

   Pages i-xxii

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2. Experimental Insect Flight Systems: Historical Contributions

   1. Front Matter

      Pages 1-1

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   2. The Contributions of Genetics to the Study of Insect Flight Muscle Function

      • Richard M. Cripps

      Pages 2-15

   3. 3D Structure of Myosin Crossbridges in Insect Flight Muscle

      • Mary C. Reedy

      Pages 16-33

   4. Comparative Physiology of Insect Flight Muscle

      • Robert K. Josephson

      Pages 34-43

   5. Stretch Activation

      • Jeffrey R. Moore

      Pages 44-60

3. Components of the Myofibril

   1. Front Matter

      Pages 61-61

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   2. Myosin

      • Becky M. Miller, Sanford I. Bernstein

      Pages 62-75

   3. Paramyosin and Miniparamyosin

      • Margarita Cervera, Juan Jose Arredondo, Raquel Marco Ferreres

      Pages 76-85

   4. Novel Myosin Associated Proteins

      • Byron Barton, Jim O. Vigoreaux

      Pages 86-96

   5. Structure of the Insect Thick Filaments

      • Gernot Beinbrech, Gereon Ader

      Pages 97-109

   6. Actin and Arthrin

      • John C. Sparrow

      Pages 110-125

   7. Troponin, Tropomyosin and GST-2

      • Alberto Ferrús

      Pages 126-140

   8. The Thin Filament in Insect Flight Muscle

      • Kevin R. Leonard, Belinda Bullard

      Pages 141-149

   9. The Insect Z-Band

      • Judith D. Saide

      Pages 150-166

   10. Projectin, the Elastic Protein of the C-Filaments

       • Agnes Ayme-Southgate, Richard Southgate

       Pages 167-176

   11. Some Functions of Proteins from the Drosophila sallimus (sls) Gene

       • Belinda Bullard, Mark C. Leake, Kevin Leonard

       Pages 177-186

4. Towards a Systems Level Analysis of Muscle

   1. Front Matter

      Pages 187-187

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   2. Sustained High Power Performance

      • Vivek Vishnudas, Jim O. Vigoreaux

      Pages 188-196

   3. X-Ray Diffraction of Indirect Flight Muscle from Drosophila in Vivo

      • Thomas C. Irving

      Pages 197-213

Methods for Obtaining X-Ray Diffraction Patterns from Drosophila 198 Diffraction Patterns from Drosophila IFM 203 Concluding Remarks 211 Note Added in Proof 211 17. Functional and Ecological Effects of Isoform Variation in Insect Flight Muscle 214 James H. Marden Abstract 214 Introduction 215 Nature's Versatile Engine 215 The Underlying Genetics: An Underinflated Genome and a Hyperinflated Transcriptome and Proteome 216 Functional Effects of Isoform Variation 219 Alternative Splicing and the Generation of Combinatorial Complexity 220 Functional Consequences of Naturally Occurring Isoform Variation 220 18. Muscle Systems Design and Integration 230 Fritz- OlafLehmann Abstract 230 Power Requirements for Flight 230 Power Reduction 233 Power Constraints on Steering Capacity 234 Balancing Power and Control 236 Changes in Muscle Efficiency in Vivo 238 Concluding Remarks 239 From the Inside Out 19. Molecular Assays for Acto-Myosin Interactions 242 John C. Sparrow and Michael A. Geeves Abstract 242 Introduction 242 Myosin Purification and Preparation of the SI Fragment 243 Purification of Flight Muscle Actin 244 Assays of Myosin and Acto-Myosin 244 Major Conclusions Relating to the Enzymatic Properties of Insect Flight Muscle Acto-Myosin 247 Major Questions about Insect Flight Muscle Acto-Myosin Kinetics That Remain 249 20.

• Biophysics
• Proteomics
• X-ray
• enzymes
• genes
• physiology
• proteins

• Department of Biology, University of Vermont, Burlington, USA

  Jim O. Vigoreaux

• Department of Molecular Physiology and Biophysics Cell and Molecular Biology Program, University of Vermont, Burlington, USA

  Jim O. Vigoreaux

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