Mass Spectrometry and Genomic Analysis

  • J. Nicholas Housby

Part of the Focus on Structural Biology book series (FOSB, volume 2)

Table of contents

  1. Front Matter
    Pages i-xv
  2. L. A. Haff, A. C. Belden, L. R. Hall, P. L. Ross, I. P. Smirnov
    Pages 16-32
  3. S. Sauer, D. Lechner, I. G. Gut
    Pages 50-65
  4. P. E. Jackson, M. D. Friesen, J. D. Groopman
    Pages 76-92
  5. W. V. Bienvenut, M. Müller, P. M. Palagi, E. Gasteiger, M. Heller, E. Jung et al.
    Pages 93-145
  6. Back Matter
    Pages 147-149

About this book

Introduction

76 2. Short Oligonucleotide Mass Analysis 76 2. 1. Method Outline 76 2. 2. Design of PCR Primers and Fragments for Analysis 78 2. 3. Typical PCR Reaction Conditions 79 3. Electrospray Ionisation Mass Spectrometry 79 Formation of Ions 3. 1. 79 3. 2. Tandem Mass Spectrometry 79 3. 3. Typical ESI-MS Settings for SOMA 80 4. Purification Procedures 80 4. 1. Phenol/Chloroform Extraction and Ethanol Precipitation 80 4. 2. In-line HPLC Purification 81 5. Genotyping Using SOMA 81 5. 1. APC Genotyping in Human Subjects 81 5. 2. APC Genotyping in Min Mice 85 5. Mutation Detection Using SOMA 86 6. 1. Analysis of p53 Mutations in Liver Cancer Patients 86 6. 1. 1. p53 Mutations in Liver Tumours 87 6. 1. 2. p53 Mutations in Plasma Samples 88 7. Advantages and Disadvantages of SOMA 89 8. Future Perspectives 90 9. Acknowledgements 91 10. References 91 CHAPTER 7 WV. Bienvenut, M. Müller, PM. Palagi, E. Gasteiger, M. Heller, E. Jung, M. Giron, R. Gras, S. Gay, PA. Binz, G J. Hughes, JC. Sanchez, RD. Appel, DF. Hochstrasser Proteomics and Mass Spectrometry: Some Aspects and Recent Developments 1. Introduction to Proteomics 93 2. Protein Biochemical and Chemical Processing Followed by Mass Spectrometric Analysis 94 2. 1. 2-DE Gel Protein Separation 95 Protein Identification Using Peptide Mass Fingerprinting and Robots 96 2. 2. 2. 2. 1. MALDI-MS Analysis 98 2. 2. 2. MS/MS Analysis 102 Improvement of the Identification by Chemical Modification of Peptides 106 2. 2. 3.

Keywords

Allele Base DNA Mass Spectrometry Nucleotide PCR SNP Single Nucleotide Polymorphism Termination genes

Editors and affiliations

  • J. Nicholas Housby
    • 1
  1. 1.Oxagen LimitedAbingdonUK

Bibliographic information

  • DOI https://doi.org/10.1007/0-306-47595-2
  • Copyright Information Kluwer Academic Publishers 2001
  • Publisher Name Springer, Dordrecht
  • eBook Packages Springer Book Archive
  • Print ISBN 978-0-7923-7173-1
  • Online ISBN 978-0-306-47595-5
  • Series Print ISSN 1571-4853
  • About this book