Animal Cell Technology: Basic & Applied Aspects

1. Growth, Immortality and Death of Cells in Culture: Present and Future Impact for the Development of Health Care Products

   1. Development of Thrombopoietin: Its Structure and Function

      • K. Ohgami

      Pages 113-117

2. Gene Transfer to Cells and Tissues of Living Animals: Applica- tion to Production and Therapeutics

   1. Baculovirus not only as an Insect Expression Vector but as a Gene Trasfer Vector

      • Jun Kobayashi

      Pages 119-123

   2. In Vivo Gene Electroporation: Manipulations of Cells to Whole Body of Living Animals

      • T. Muramatsu

      Pages 125-129

3. Cell Culture for Assessment of Physiologically Functional Substances

   1. In Vitro Stimulation of Tumor Necrosis Factor Production from Rat Splenocytes by Cabbage Extract

      • Wataru Komatsu, Yutaka Miura, Kazumi Yagasaki

      Pages 131-134

   2. Effects of Green, Oolong and Black Teas and Related Components on Proliferation and Invasion of Hepatoma Cells

      • G. Y. Zhang, Y. Miura, K. Yagasaki

      Pages 135-139

4. Expression of Recombinant Animal Genes in Cultured Animal Cells

   1. Milk Secretion in Cultured Mammary Epithelial Cells

      • David R Blatchford, Colin J. Wilde, T. Matsuda, N Aoki

      Pages 141-145

   2. Hyperthermic Induction of Gene Expression Using Stress Inducible Promoter

      • I. A. Bouhon, A. Ito, M. Shinkai, H. Honda, T. Kobayashi

      Pages 147-152

5. Cell Culture Engineering

   1. Enhancement of Productivity of Recombinant α-Amidating Enzyme by Low Temperature Culture

      • K. Furukawa, K. Ohsuye

      Pages 153-157

   2. Dialysis-fixed-bed Cultures for Highly Efficient Production of Chimeric Fab-fragments with Transfec Toma Cells in Protein-free Medium

      • R. Pörtner, I. Lüdemann, K. Reher, M. Neumaier, H. MÄrkl

      Pages 159-163

6. Cost Performance for Producing Biochemicals

   1. Monitoring Drug Effects on Human Primary Tissues Utilizing Fibre Otical Sensors

      • H. Maerz, R. Buchholz, F. Emmrich, F. Fink, L. Pfeifer, U. Marx

      Pages 165-169

7. Establishment of Functional Cell Lines for Production of Biologicals

   1. Increasing Antibody Production by Suppressing Cell Proliferation with Controllable Expression of Cyclin Inhibitor P21 and P27

      • Tetsuo Fujita, Satoshi Terada, Hiroshi Ueda, Teruyuk Nagamune, Eiji Suzuki

      Pages 171-175

   2. Establishment of an Apoptosis-Resistant and Growth-Controllable Cell Line by Transfecting With Inducible Antisense c-Jun Gene

      • Yon Hui Kim, Takehiro Iida, Edward V. Prochownik, Eiji Suzuki

      Pages 177-184

   3. Improvement of Cell Sirvoval by Inhibition of Caspases Playing Key Roles in Apoptosis

      • S. Terada, T. Yata, K. Fukuoka, H. Aizama, T. Fujita, T. Nagamune et al.

      Pages 185-189

8. Application of Human Monoclonal Antibody

   1. In Vitro Immunization of Human Peripheral Blood Lymphocytes with 14–16 kDa Rice Allergen Proteins

      • A. Ichikawa, Y. Katakura, K. Teruya, S. Hashizume, T. Matsuda, S. Shirahata

      Pages 191-197

9. Cell Characterization and Validation

   1. Options to Eliminate Animal-Derived Components of Cell Culture Media

      • David W. Jayme, Shawn R. Smith

      Pages 199-203

   2. Characterization of the Subpopulations of Rat Ascites Hepatoma AH109A Cells With Different Invasive and Metastatic Activities

      • Y. Miura, M. Miyauchi, K. Kubota, Q. Li, H. Tachikawa, D. Fujimoto et al.

      Pages 205-209

   3. Functional Expression of NMDA Receptor Channels in Differentiating Neural Precursor Cells

      • K. Yamada, T. Hisatsune, S. Uchino, T. Nakamura, Y. Kudo, S. Kaminogawa

      Pages 211-215

   4. TGF-β Reversibly Induces Cell Senescence in Adenocarcinoma Cell Line

      • Yoshinori Katakura, Eriko Nakata, Takumi Miura, Sanetaka Shirahata

      Pages 217-221

10. Cell Culture Engineering

    1. Evaluation of a Fixed-Bed Type Bioreactor System Using Recombinant CHO Cells Producing Human Transferrin

       • A. Nemoto, Y. Hirota-Iwai, Y. Miyazaki, N. Nakamichi, M. Ito, T. Tokiwa et al.

       Pages 223-227

    2. Formation of Cylindrical Multicellular Aggregate (Cylindroid) of Rat Hepatocytes on Pressed Sheet of Polyurethane Foam

       • Hiroshi Mizumoto, Mitsuru Kaneko, Kohji Nakazawa, Hiroyuki Ijima, Taku Matsushita, Kazumori Funatsu

       Pages 229-233

Animal cell technology is a growing discipline of cell biology which aims not only to understand structures, functions and behaviors of differentiated animal cells but also to ascertain their abilities to be used for industrial and medical purposes. The goal of animal cell technology includes accomplishments of clonal expansion of differentiated cells with useful ability, optimization of their culture conditions, modulation of their ability for production of medically and pharmaceutically important proteins, and the application of animal cells to gene therapy and artificial organs. This Volume gives the readers a complete review of the present state of the art in Japan. The Proceedings will be useful for cell biologists, biochemists, molecular biologists, immunologists, biochemical engineers and other disciplines related to animal cell culture, working either in academic environments or in industries of biotechnology and pharmacy.

• Expression
• Transplantation
• biotechnology
• development
• gene therapy
• gene transfer
• proteins
• transgen

• Bioscience Center, Nagoya University, Nagoya, Japan

  Y. Kitagawa

• Department of Applied Biological Sciences, Graduate School of Agricultural Sciences, Nagoya University, Nagoya, Japan

  T. Matsuda

• Department of Biotechnology, Graduate School of Engineering, Nagoya University, Nagoya, Japan

  S. Iijima

Policies and ethics