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© 2019

Microfluidics for Single-Cell Analysis

  • Jin-Ming Lin
Book

Part of the Integrated Analytical Systems book series (ANASYS)

Table of contents

  1. Front Matter
    Pages i-vii
  2. Qiushi Huang, Jin-Ming Lin
    Pages 1-26
  3. Weiwei Li, Jin-Ming Lin
    Pages 53-84
  4. Weifei Zhang, Nan Li, Jin-Ming Lin
    Pages 85-117
  5. Mashooq Khan, Jin-Ming Lin
    Pages 143-161
  6. Sifeng Mao, Jin-Ming Lin
    Pages 217-255
  7. Back Matter
    Pages 257-261

About this book

Introduction

This book summarizes the various microfluidic-based approaches for single-cell capture, isolation, manipulation, culture and observation, lysis, and analysis. Single-cell analysis reveals the heterogeneities in morphology, functions, composition, and genetic performance of seemingly identical cells, and advances in single-cell analysis can overcome the difficulties arising due to cell heterogeneity in the diagnostics for a targeted model of disease. This book provides a detailed review of the state-of-the-art techniques presenting the pros and cons of each of these methods. It also offers lessons learned and tips from front-line investigators to help researchers overcome bottlenecks in their own studies. Highlighting a number of techniques, such as microfluidic droplet techniques, combined microfluidics-mass-spectrometry systems, and nanochannel sampling, it describes in detail a new microfluidic chip-based live single-cell extractor (LSCE) developed in the editor’s laboratory, which opens up new avenues to use open microfluidics in single-cell extraction, single-cell mass spectrometric analysis, single-cell adhesion analysis and subcellular operations. Serving as both an elementary introduction and advanced guidebook, this book interests and inspires scholars and students who are currently studying or wish to study microfluidics-based cell analysis methods.

Keywords

Single Cell Analysis Cell Co-culture Mass Spectrometry Microfluidics Cell Isolation and Recognition Droplet Generation

Editors and affiliations

  • Jin-Ming Lin
    • 1
  1. 1.Department of ChemistryTsinghua UniversityBeijingChina

About the editors



Bibliographic information