Molecular Diagnostics

Part 1: Technical Backgrounds and Quality Aspects

  • E. van Pelt-Verkuil
  • W.B. van Leeuwen
  • R. te Witt

Table of contents

  1. Front Matter
    Pages i-vii
  2. E. van Pelt-Verkuil, R. te Witt
    Pages 1-6
  3. E. van Pelt-Verkuil, J. W. Swierstra
    Pages 7-50
  4. E. van Pelt-Verkuil, R. te Witt
    Pages 51-95
  5. E. van Pelt-Verkuil, R. te Witt
    Pages 97-129
  6. E. van Pelt-Verkuil, R. te Witt
    Pages 131-215
  7. E. van Pelt-Verkuil, W. B. van Leeuwen
    Pages 217-319
  8. H. G. M. Niesters, W. B. van Leeuwen
    Pages 321-337
  9. C. W. Knetsch, E. M. van der Veer, C. Henkel, P. Taschner
    Pages 339-360
  10. P. Taschner, C. Henkel
    Pages 361-386
  11. E. van Pelt-Verkuil, A. Ruiz-Villalba, J. M. Ruijter
    Pages 411-440
  12. Back Matter
    Pages 441-457

About this book


The books Molecular Diagnostics Part 1 and 2 provide a comprehensive and practical overview of the state-of-the-art molecular biological diagnostic strategies that are being used in a wide variety of disciplines.

The editors and experts in their respective fields have combined their knowledge to write these two books. Many years of experience in the development, application and quality control of molecular diagnostic methods is reflected herewith.

Molecular Diagnostics Part 1 is dedicated to the theoretical backgrounds of the technologies often applied in molecular diagnostics, in which nucleic acid amplification methods (such as real-time PCR), sequencing and bioinformatics are the basic tools. The assay design and -development, combined with items of trouble-shooting are described in detail. As a foundation of reliable molecular diagnostic assays, the quality control required for validation, implementation and performance of molecular diagnostic assays is thoroughly discussed. This book also provides extensive information for those working with molecular techniques in a wide variety of research applications using conventional and real-time PCR technology, Sanger and high throughput sequencing techniques, and bioinformatics.

Molecular Diagnostics Part 2 highlights the applications of the molecular diagnostic methods in the various diagnostic laboratories, comprising:

- Clinical microbiology

- Clinical chemistry 

- Clinical genetics

- Clinical pathology 

- Molecular hematopathology

- Veterinary health

- Plant health

- Food safety

Both full-color and well-illustrated books are particularly valuable for students, clinicians, scientists and other professionals who are interested in (designing) molecular diagnostic methods and for those who wish to broaden their knowledge on the current molecular biological revolution. The information in the books highlights the trend of the integration of multiple (clinical) disciplines into one universal molecular laboratory. 


Disease Characterization Real-Time PCR Amplification Techniques Quality Control Next Gen Sequencing

Editors and affiliations

  • E. van Pelt-Verkuil
    • 1
  • W.B. van Leeuwen
    • 2
  • R. te Witt
    • 3
  1. 1.Leiden Centre for Applied Science, Faculty of Science & TechnologyUniversity of Applied Sciences LeidenLeidenThe Netherlands
  2. 2.Leiden Centre for Applied Science, Faculty of Science & TechnologyUniversity of Applied Sciences LeidenLeidenThe Netherlands
  3. 3.NMDL-LCPLRijswijkThe Netherlands

Bibliographic information