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Study of Bacteriorhodopsin in a Controlled Lipid Environment

  • Vivien Yeh
Book
  • 1.1k Downloads

Part of the Springer Theses book series (Springer Theses)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Vivien Yeh
    Pages 1-36
  3. Vivien Yeh
    Pages 37-65
  4. Vivien Yeh
    Pages 93-111
  5. Vivien Yeh
    Pages 113-133
  6. Vivien Yeh
    Pages 135-137
  7. Back Matter
    Pages 139-139

About this book

Introduction

This book focuses on the study of how the properties of nanodiscs, such as lipid composition and size, influence the function of the embedding integral membrane protein, bacteriorhodopsin. The author performed systematic studies to show that the lipid composition and the charge of the hydrophobic head and the structure of hydrophilic tails affect the photocycle pathway of bacteriorhodopsin, which is closely associated with its proton-pumping activity. Furthermore, the author demonstrated a highly efficient method for extracting membrane proteins directly from the biological membrane, preserving protein conformation, function and essential native lipids. This book demonstrates optimization and sample preparation, and presents practical methods of preparing membrane protein-embedded nanodisc samples for biophysical studies, which benefit structural and functional studies in the field of membrane protein characterization, both.

Keywords

Membrane protein Lipid nanodisc Bacteriorhodopsin Photocycle kinetics Lipid composition Native membrane Circular membrane scaffold protein

Authors and affiliations

  • Vivien Yeh
    • 1
  1. 1.Department of ChemistryNational Taiwan UniversityTaipeiTaiwan

Bibliographic information