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Enzyme-linked Immunosorbent Assay (ELISA)

From A to Z

  • Samira Hosseini
  • Patricia Vázquez-Villegas
  • Marco Rito-Palomares
  • Sergio O. Martinez-Chapa

Part of the SpringerBriefs in Applied Sciences and Technology book series (BRIEFSAPPLSCIENCES)

Also part of the SpringerBriefs in Forensic and Medical Bioinformatics book sub series (BRIEFSFOMEBI)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Samira Hosseini, Patricia Vázquez-Villegas, Marco Rito-Palomares, Sergio O. Martinez-Chapa
    Pages 1-18
  3. Samira Hosseini, Patricia Vázquez-Villegas, Marco Rito-Palomares, Sergio O. Martinez-Chapa
    Pages 19-29
  4. Samira Hosseini, Patricia Vázquez-Villegas, Marco Rito-Palomares, Sergio O. Martinez-Chapa
    Pages 57-66
  5. Samira Hosseini, Patricia Vázquez-Villegas, Marco Rito-Palomares, Sergio O. Martinez-Chapa
    Pages 67-115

About this book

Introduction

This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices. 

Keywords

Side Chain Theory Antigen-antibody Binding Theory Radioimmunoassay (RIA) Antibody Production Humoral Immunity Cancer Detection Antispecies Antibodies Enzyme Conjugation Chemifluorescenct Assay Covalent Immobilization Plasmonic ELISA ELISA in Microdevices

Authors and affiliations

  • Samira Hosseini
    • 1
  • Patricia Vázquez-Villegas
    • 2
  • Marco Rito-Palomares
    • 3
  • Sergio O. Martinez-Chapa
    • 4
  1. 1.Escuela de Ingeniería y CienciasTecnologico de MonterreyMonterreyMexico
  2. 2.Escuela de Ingeniería y CienciasTecnologico de MonterreyMonterreyMexico
  3. 3.Escuela de Medicina y Ciencias de SaludTecnologico de MonterreyMonterreyMexico
  4. 4.Escuela de Ingeniería y CienciasTecnologico de MonterreyMonterreyMexico

Bibliographic information

  • DOI https://doi.org/10.1007/978-981-10-6766-2
  • Copyright Information The Author(s) 2018
  • Publisher Name Springer, Singapore
  • eBook Packages Engineering
  • Print ISBN 978-981-10-6765-5
  • Online ISBN 978-981-10-6766-2
  • Series Print ISSN 2191-530X
  • Series Online ISSN 2191-5318
  • Buy this book on publisher's site