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Biological Small Angle Scattering: Techniques, Strategies and Tips

  • Barnali Chaudhuri
  • Inés G. Muñoz
  • Shuo Qian
  • Volker S. Urban

Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 1009)

Table of contents

  1. Front Matter
    Pages i-x
  2. Melissa A. Graewert, Cy M. Jeffries
    Pages 11-30
  3. Martha Brennich, Petra Pernot, Adam Round
    Pages 47-64
  4. Maxim V. Petoukhov, Anne Tuukkanen
    Pages 87-105
  5. Tiago N. Cordeiro, Fátima Herranz-Trillo, Annika Urbanek, Alejandro Estaña, Juan Cortés, Nathalie Sibille et al.
    Pages 107-129
  6. Yujing Wang, Hao Zhou, Emre Onuk, John Badger, Lee Makowski
    Pages 131-147
  7. Carlotta Marasini, Bente Vestergaard
    Pages 149-165
  8. Henry Y. H. Tang, John A. Tainer, Greg L. Hura
    Pages 167-181
  9. Javier Pérez, Patrice Vachette
    Pages 183-199
  10. An Hsieh, Lanyuan Lu, Mark R. Chance, Sichun Yang
    Pages 229-238
  11. Aaron Alford, Veronika Kozlovskaya, Eugenia Kharlampieva
    Pages 239-262
  12. Back Matter
    Pages 263-268

About this book

Introduction

This book provides a clear, comprehensible and up-to-date description of how Small Angle Scattering (SAS) can help structural biology researchers. SAS is an efficient technique that offers structural information on how biological macromolecules behave in solution. SAS provides distinct and complementary data for integrative structural biology approaches in combination with other widely used probes, such as X-ray crystallography, Nuclear magnetic resonance, Mass spectrometry and Cryo-electron Microscopy. The development of brilliant synchrotron small-angle X-ray scattering (SAXS) beam lines has increased the number of researchers interested in solution scattering. SAS is especially useful for studying conformational changes in proteins, highly flexible proteins, and intrinsically disordered proteins. Small-angle neutron scattering (SANS) with neutron contrast variation is ideally suited for studying multi-component assemblies as well as membrane proteins that are stabilized in surfactant micelles or vesicles. SAS is also used for studying dynamic processes of protein fibrillation in amyloid diseases, and pharmaceutical drug delivery. The combination with size-exclusion chromatography further increases the range of SAS applications.

The book is written by leading experts in solution SAS methodologies. The principles and theoretical background of various SAS techniques are included, along with practical aspects that range from sample preparation to data presentation for publication. Topics covered include techniques for improving data quality and analysis, as well as different scientific applications of SAS. With abundant illustrations and practical tips, we hope the clear explanations of the principles and the reviews on the latest progresses will serve as a guide through all aspects of biological solution SAS.

The scope of this book is particularly relevant for structural biology researchers who are new to SAS. Advanced users of the technique will find it helpful for exploring the diversity of solution SAS methods and applications.

Chapter 3 of this book is available open access under a CC BY 4.0 license at link.springer.com.

Keywords

SAS SAXS SANS small angle neutron scattering small angle X-ray scattering

Editors and affiliations

  • Barnali Chaudhuri
    • 1
  • Inés G. Muñoz
    • 2
  • Shuo Qian
    • 3
  • Volker S. Urban
    • 4
  1. 1.G N Ramachandran Protein CenterCSIR Institute of Microbial TechnologyChandigarhIndia
  2. 2.Structural Biology ProgrammeSpanish National Cancer Research Centre (CNIO)MadridSpain
  3. 3.Center for Structural Molecular Biology and Neutron Scattering DivisionOak Ridge National LaboratoryOak RidgeUSA
  4. 4.Center for Structural Molecular Biology and Neutron Scattering DivisionOak Ridge National LaboratoryOak RidgeUSA

Bibliographic information

  • DOI https://doi.org/10.1007/978-981-10-6038-0
  • Copyright Information Springer Nature Singapore Pte Ltd. 2017
  • Publisher Name Springer, Singapore
  • eBook Packages Biomedical and Life Sciences
  • Print ISBN 978-981-10-6037-3
  • Online ISBN 978-981-10-6038-0
  • Series Print ISSN 0065-2598
  • Series Online ISSN 2214-8019
  • Buy this book on publisher's site