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DNA Cloning: A Hands-on Approach

  • Seok-Yong Choi
  • Hyunju Ro
  • Hankuil Yi
Book

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Seok-Yong Choi, Hyunju Ro, Hankuil Yi
    Pages 1-4
  3. Seok-Yong Choi, Hyunju Ro, Hankuil Yi
    Pages 5-28
  4. Seok-Yong Choi, Hyunju Ro, Hankuil Yi
    Pages 29-66
  5. Seok-Yong Choi, Hyunju Ro, Hankuil Yi
    Pages 67-77
  6. Seok-Yong Choi, Hyunju Ro, Hankuil Yi
    Pages 79-98
  7. Seok-Yong Choi, Hyunju Ro, Hankuil Yi
    Pages 99-121
  8. Seok-Yong Choi, Hyunju Ro, Hankuil Yi
    Pages 123-125
  9. Back Matter
    Pages 127-131

About this book

Introduction

This book offers step-by-step instruction on DNA cloning, defined as moving genes around plasmids, mutating genes, or mining new genes. The aim is to provide those new to the field with reliable and up-to-date practical guidance while at the same time conveying the scope for creativity. After a brief synopsis of the history of cloning, the fundamentals and prerequisites are explained, covering, for example, software, vectors commonly used in the lab, appropriate choice of restriction endonucleases, the preparation of agarose gels, competent cells, and LB agar plates, and procedures to be followed upon receipt of new plasmids. The remainder of the book is devoted to the clear description of methods and individual steps in cloning. Guidance is provided on the cut and paste method, DNA sequencing, direct sequencing, primer design, PCR-based gene insertion and deletion, epitope tag insertion, the use of RACE technology, BAC recombineering, and much, much more. Sources of error and a variety of techniques that make life considerably easier when cloning are also examined in detail. 

Keywords

Cloning DNA PCR plasmid vector

Authors and affiliations

  • Seok-Yong Choi
    • 1
  • Hyunju Ro
    • 2
  • Hankuil Yi
    • 3
  1. 1.Department of Biomedical SciencesChonnam National University Medical SchoolGwangjuKorea (Republic of)
  2. 2.Department of Biological SciencesChungnam National UniversityDaejeonKorea (Republic of)
  3. 3.Department of Biological SciencesChungnam National UniversityDaejeonKorea (Republic of)

Bibliographic information