Plant Cell and Tissue Culture

  • Indra K. Vasil
  • Trevor A. Thorpe

Table of contents

  1. Front Matter
    Pages i-x
  2. Methodology

    1. Front Matter
      Pages 1-1
    2. Friedrich Constabel, Jerry P. Shyluk
      Pages 3-15
    3. Trevor A. Thorpe
      Pages 17-36
    4. Narender S. Nehra, Kutty K. Kartha
      Pages 37-70
    5. G. Wenzel, B. Foroughi-Wehr
      Pages 153-172
    6. K. K. Kartha, F. Engelmann
      Pages 195-230
    7. M. A. W. Hinchee, D. R. Corbin, Ch. L. Armstrong, J. E. Fry, S. S. Sato, D. L. DeBoer et al.
      Pages 231-270
    8. F. Constabel, R. T. Tyler
      Pages 271-289
  3. In vitro Cultures

    1. Front Matter
      Pages 291-291
    2. Indra K. Vasil, Vimla Vasil
      Pages 293-312
    3. M. R. Davey, V. Kumar, N. Hammatt
      Pages 313-329
    4. John F. Reynolds
      Pages 331-362
    5. M. G. K. Jones
      Pages 363-378
    6. Abraham D. Krikorian
      Pages 379-411
    7. C. E. Palmer, W. A. Keller
      Pages 413-455
    8. Richard H. Zimmerman, Harry J. Swartz
      Pages 457-474
    9. J. W. Grosser
      Pages 475-496
    10. Abraham D. Krikorian
      Pages 497-537
    11. Indra S. Harry, Trevor A. Thorpe
      Pages 539-560
    12. Pierre Debergh
      Pages 561-573
  4. Back Matter
    Pages 575-593

About this book


Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops.
Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites.
Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals.
Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field.


DNA Embryo Tree cell lines cryopreservation roots

Editors and affiliations

  • Indra K. Vasil
    • 1
  • Trevor A. Thorpe
    • 2
  1. 1.Laboratory of Plant Cell and Molecular Biology, Department of Horticultural SciencesUniversity of FloridaGainesvilleUSA
  2. 2.Plant Physiology Research Group, Department of Biological SciencesThe University of CalgaryCalgaryCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media B.V. 1994
  • Publisher Name Springer, Dordrecht
  • eBook Packages Springer Book Archive
  • Print ISBN 978-90-481-4327-6
  • Online ISBN 978-94-017-2681-8
  • Buy this book on publisher's site