DNA Methods in Clinical Microbiology

  • Authors
  • Paul Singleton

Table of contents

  1. Front Matter
    Pages i-vii
  2. Paul Singleton
    Pages 1-11
  3. Paul Singleton
    Pages 44-55
  4. Paul Singleton
    Pages 126-151
  5. Paul Singleton
    Pages 168-202
  6. Paul Singleton
    Pages 229-236
  7. Back Matter
    Pages 237-255

About this book


DNA Methods in Clinical Microbiology describes the novel DNA-based technology now used in the diagnosis and management of infectious diseases. It is a concise, yet readable, overview written primarily for clinicians, clinical microbiologists, medical students and undergraduates in medical and veterinary microbiology.
The book has two primary aims. First, to explain the principles of these methods at the `molecular' level. Second, to provide a clinical perspective by reporting results from actual DNA-based investigations on a range of specimens.
Those approaching DNA methods for the first time are assisted by a brief résumé of the relevant features of nucleic acids (Chapter 2): this information is essential for an understanding of later chapters. Subsequent text covers detection, characterization and quantification of pathogens by a variety of methods - e.g., target amplification (PCR, LCR, NASBA, TMA and SDA), signal amplification (bDNA) and probe-based techniques; the chapter on typing describes nearly twenty named molecular methods, including spoligotyping and MLST. All chapters include an adequate range of current reference from which, if required, detailed protocols can be obtained. The diagrams are clear, and readers are assisted by a detailed index.


DNA antibiotic biology diagnosis infectious disease microbiology nucleic acid polymer

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media B.V. 2000
  • Publisher Name Springer, Dordrecht
  • eBook Packages Springer Book Archive
  • Print ISBN 978-90-481-5456-2
  • Online ISBN 978-94-017-1286-6
  • Buy this book on publisher's site