Cell Culture Methods for In Vitro Toxicology

  • Glyn N. Stacey
  • Alan Doyle
  • Margherita Ferro

Table of contents

  1. Front Matter
    Pages i-ix
  2. Margherita Ferro, Alan Doyle
    Pages 1-8
  3. Glyn Stacey, Caroline MacDonald
    Pages 27-42
  4. Bruce Jones, Glyn Stacey
    Pages 43-66
  5. Thierry Battle, Glyn Stacey
    Pages 83-95
  6. Yula Sambruy, S. Ferruzza, G. Ranaldi, I. De Angelis
    Pages 97-113
  7. Glyn Stacey, Barbara Viviani
    Pages 115-130
  8. Michel Fischbach, Peter Bromley
    Pages 131-147
  9. Back Matter
    Pages 149-153

About this book


Cell Culture Methods for in vitro Toxicology introduces the reader to a range of techniques involved in the use of in vitro cell culture in toxicological studies. It deals with major cell types studied in the field of toxicology and will be useful for anyone wishing to start work with animal cell cultures or to refresh their knowledge relating to in vitro cell models. Fundamental chapters deal with the general biology of cytotoxicity and cell immortalisation these are key issues for in vitro systems addressing the `3Rs' principle. Up-to-date overviews deal with the use of cells from liver, brain and intestine. In addition, biochemical analysis of cell responses, biotransformation pathways in cells and recombinant approaches to the early detection of cell stress are also covered in detail.
Prominent features of in vitro technologies also include regulation, biosafety and standardisation. Dedicated chapters deal with these issues in a practical way in order to lead the reader to the right source of information. This book provides an up-to-date, informative and practical review of cell culture methods for in vitro toxicology. It will be of equal benefit to students and experienced toxicologists with little experience of in vitro cell culture.


biology drug lead neurotoxicology pharmacology research system toxicity toxicology

Editors and affiliations

  • Glyn N. Stacey
    • 1
  • Alan Doyle
    • 2
  • Margherita Ferro
    • 3
  1. 1.National Institute of Biological Standards and ControlPotters BarUK
  2. 2.The Wellcome TrustLondonUK
  3. 3.University of GenovaItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media B.V. 2001
  • Publisher Name Springer, Dordrecht
  • eBook Packages Springer Book Archive
  • Print ISBN 978-90-481-5936-9
  • Online ISBN 978-94-017-0996-5
  • Buy this book on publisher's site