Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology

Selected articles from the Meeting of the EFB Section on Microbial Physiology, Semmering, Austria, 5th–8th October 2000

  • O.-W. Merten
  • D. Mattanovich
  • C. Lang
  • G. Larsson
  • P. Neubauer
  • D. Porro
  • P. Postma
  • J. Teixeira de Mattos
  • J. A. Cole

Table of contents

  1. Front Matter
    Pages i-x
  2. Cell-free translation systems

  3. Metabolic burden and stress response

    1. Randal J. Kaufman, Donalyn Scheuner, Witoon Tirasophon, Chuan Yin-Liu, Benbo Song, Kuei C. Lee et al.
      Pages 17-27
    2. Matthew P. DeLisa, Ryan T. Gill, William E. Bentley
      Pages 43-54
    3. Hong Ying Lin, Renate Hanschke, Silke Nicklisch, Stephan Riemschneider, Sylke Meyer, Antje Gupta et al.
      Pages 55-73
    4. Pauli T. Kallio, Alexander D. Frey, James E. Bailey
      Pages 75-87
  4. Genetic stability and gene copy number effects

    1. R. Kunert, K. Strutzenberger, F. Steindl, A. Zudjelovic, N. Borth, H. Katinger
      Pages 89-97
    2. Julian A. J. Hanak, Rocky M. Cranenburgh
      Pages 111-124
    3. Joseph Boudrant, Baolinh Le, Frantz Fournier, Christian Fonteix
      Pages 125-139
  5. Transcription, translation, and product formation in E. coli

    1. Dean E. McNulty, Michael J. Huddleston, Brett Claffee, Susan Green, Ganesh Sathe, Robert Reeves et al.
      Pages 151-158
    2. Nina Q. Meinander, Marie Jeppsson, Morten Sögaard
      Pages 159-170
    3. Thomas Nyström, Manuel Ballesteros, Åsa Fredriksson
      Pages 171-178
    4. Gerald Striedner, Monika Cserjan-Puschmann, Reingard Grabherr, Franz Clementschitsch, Erik Nilsson, Karl Bayer
      Pages 179-188
  6. Transcription, translation, and product formation in microbial systems other than E. coli

    1. A. Serrano, G. Lin Cereghino, P. Ferrer, J. M. Cregg, F. Valero
      Pages 259-267
    2. L. Bredmose, S. M. Madsen, A. Vrang, P. Ravn, M. G. Johnsen, J. Glenting et al.
      Pages 269-275
  7. Animal cell based expression systems — process optimisation

  8. Comparison of different microbial expression systems

  9. Back Matter
    Pages 393-400

About this book


More then 20 years have passed now since the first recombinant protein producing microorganisms have been developed. In the meanwhile, numerous proteins have been produced in bacteria, yeasts and filamentous fungi, as weIl as higher eukaryotic cells, and even entire plants and animals. Many recombinant proteins are on the market today, and some of them reached substantial market volumes. On the first sight one would expect the technology - including the physiology of the host strains - to be optimised in detail after a 20 year's period of development. However, several constraints have limited the incentive for optimisation, especially in the pharmaceutical industry like the urge to proceed quickly or the requirement to define the production parameters for registration early in the development phase. The additional expenses for registration of a new production strain often prohibits a change to an optimised strain. A continuous optimisation of the entire production process is not feasible for the same reasons.


Bur Codons DNA Fermentation Mammalia Metabolism Oxidation Translation biotechnology development gene expression genes microorganism proteins transcription

Editors and affiliations

  • O.-W. Merten
    • 1
  • D. Mattanovich
    • 2
  • C. Lang
    • 3
  • G. Larsson
    • 4
  • P. Neubauer
    • 5
  • D. Porro
    • 6
  • P. Postma
    • 7
  • J. Teixeira de Mattos
    • 8
  • J. A. Cole
    • 9
  1. 1.Genethon IIIEvry-CedexFrance
  2. 2.Institute for Applied MicrobiologyUniversity of Agricultural SciencesWienAustria
  3. 3.Institute of BiotechnologyTechnical University of BerlinBerlinGermany
  4. 4.The Swedish Centre on Bioprocess TechnologyKTHStockholmSweden
  5. 5.Institute of BiotechnologyM. Luther University of Halle-WittenbergHalleGermany
  6. 6.Department of Biotechnology and BiosciencesUniversity of Milano-BicoccaMilanItaly
  7. 7.E.C. Slater InstituteUniversity of AmsterdamThe Netherlands
  8. 8.Swammerdam Institute of Life SciencesUniversity of AmsterdamThe Netherlands
  9. 9.School of BiosciencesUniversity of BirminghamUK

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media B.V. 2001
  • Publisher Name Springer, Dordrecht
  • eBook Packages Springer Book Archive
  • Print ISBN 978-90-481-5756-3
  • Online ISBN 978-94-015-9749-4
  • Buy this book on publisher's site