Safety in Cell and Tissue Culture

  • G. Stacey
  • A. Doyle
  • P. Hambleton

Table of contents

  1. Front Matter
    Pages i-ix
  2. Glyn Stacey, Alan Doyle, David Tyrrell
    Pages 1-25
  3. Trevor Littlewood
    Pages 26-48
  4. Alan Jennings
    Pages 49-63
  5. Bruce Jones
    Pages 64-86
  6. Alan Doyle, Glyn Stacey
    Pages 102-115
  7. John Benbough, B. Andrew Curran
    Pages 116-130
  8. Bryan Griffiths, Wolfgang Noe
    Pages 135-154
  9. Heather Sheeley
    Pages 173-188
  10. Caroline MacDonald
    Pages 189-204
  11. Back Matter
    Pages 223-244

About this book


It is now more than half a century since animal cells first came into regular use in the laboratory. Instances of laboratory acquired infection and con­ tamination of therapeutic products, derived from the use of animal cell cultures are rare. The use of animal cells, in addition to an established role in the production of vaccines and therapeutic proteins, has many new medical applications including gene therapy, tissue engineering and cell therapy. Furthermore, C;ldvances in molecular and cell biology are enabling rapid development and application of these technologies and the development of new and more sensitive methods, such as nucleic acid amplification, for the characterisation of cells and the detection of adven­ titious agents. However, it is clear that there is no room for complacency in this field and the recent expansion in the use of animal cells in the manufacture of medical products and the development of new biological assays for diagnostic and pharmaco-toxicological screening, underlines the need for vigilance regarding the correct and safe use of animal cells as substrates. This book is therefore very timely and should prove to be a highly valuable text, finding a wider audience beyond those with respon­ sibility for laboratory safety. The book guides the reader from fundamental cell biology issues and the establishment of new in vitro methods, through testing and validation of cell lines and on to issues in the use of animal cells in manufacturing processes.


biological materials biology cell biology classification microorganism tissue

Editors and affiliations

  • G. Stacey
    • 1
  • A. Doyle
    • 2
  • P. Hambleton
    • 3
  1. 1.National Institute for Biological Standards and ControlSouth Mimms, HertfordshireUK
  2. 2.Centre for Applied Microbiology and ResearchEuropean Collection of Cell CulturesSalisbury, WiltshireUK
  3. 3.Centre for Applied Microbiology and ResearchSalisbury, WiltshireUK

Bibliographic information