© 2000

Plant Molecular Biology Manual

  • Stanton B. Gelvin
  • Robbert A. Schilperoort

Table of contents

  1. Front Matter
    Pages i-xxi
  2. Annemarie H. Meijer, Jan Schouten, Pieter B. F. Ouwerkerk, J. Harry C. Hoge
    Pages 29-57
  3. L. Alexander Lyznik, David Peterson, Zuo-Yu Zhao, Xueni Guan, Ben Bowen, Bruce Drummond et al.
    Pages 75-99
  4. Christopher J. Staiger, Bryan C. Gibbon, Haiyun Ren
    Pages 101-116
  5. Chiyoko Machida, Hitoshi Onouchi, Endang Semiarti, Takaaki Ishikawa, Yasunori Machida
    Pages 117-141
  6. Back Matter
    Pages 143-167

About this book


Plant Molecular Biology Manual (Second Edition) is an entirely new manual containing both fundamental and recently described techniques in the area of plant molecular biology. Designed for use in the research laboratory, the Plant Molecular Biology Manual presents detailed techniques in the areas of plant transformation, recombinant DNA and other nucleic acid manipulations, nuclear run-on and in vitro transcription systems, in situ hybridization and immunodetection systems, protein-nucleic acid interaction analyses, subcellular targeting of proteins in the plant cell, and gene tagging using T-DNA and transposons. This second edition contains more than 40 newly written chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized Agrobacterium strains and T-DNA vectors, nuclear run-on and in vitro transcription systems, non-radioactive detection systems, characterization of transcription factors, nuclear protein targeting, and T-DNA and transposon mutagenesis, not previously described in the first edition.


Chloroplast DNA Expression PCR YAC arabidopsis thaliana biology gene expression gene transfer genetic engineering hybridization molecular biology plant protein transcription

Editors and affiliations

  • Stanton B. Gelvin
    • 1
  • Robbert A. Schilperoort
    • 2
  1. 1.Purdue UniversityWest LafayetteUSA
  2. 2.Leiden UniversityLeidenThe Netherlands

Bibliographic information

  • Book Title Plant Molecular Biology Manual
  • Editors S.B. Gelvin
    R.A. Schilperoort
  • DOI
  • Copyright Information Springer Science+Business Media Dordrecht 2000
  • Publisher Name Springer, Dordrecht
  • eBook Packages Springer Book Archive
  • Softcover ISBN 978-94-011-7655-2
  • eBook ISBN 978-94-011-4217-5
  • Edition Number 2
  • Number of Pages XXI, 161
  • Number of Illustrations 0 b/w illustrations, 0 illustrations in colour
  • Topics Plant Sciences
    Plant Physiology
  • Buy this book on publisher's site


About the first edition:
` Altogether, Plant Molecular Biology Manual is a very useful guide for the practical application of modern methods of molecular biology in plant sciences and is therefore highly recommended. '
Plant Breeding
` ...every researcher in plant molecular biology should take a look at this book '
New Phytologist