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Human Cancer in Primary Culture, A Handbook

  • J. R. W. Masters

Part of the Developments in Oncology book series (DION, volume 64)

Table of contents

  1. Front Matter
    Pages i-x
  2. Part One

    1. Front Matter
      Pages 1-1
    2. Adi F. Gazdar, Jeffrey M. Trent
      Pages 3-27
    3. Paul Collodi, Cathleen Rawson, David Barnes
      Pages 29-53
    4. Norbert E. Fusenig, Dirk Breitkreutz, Petra Boukamp
      Pages 55-80
    5. Einar K. Rofstad
      Pages 81-101
    6. Larry M. Weisenthal
      Pages 103-147
  3. Part Two

    1. Front Matter
      Pages 149-149
    2. Christos Paraskeva, Angela Hague
      Pages 151-168
    3. Martin F. Pera
      Pages 169-185
    4. E. Kenneth Parkinson, W. Andrew Yeudall
      Pages 187-197
    5. Peter R. Twentyman
      Pages 199-229
    6. John L. Darling
      Pages 231-251
    7. Richard D. H. Whelan, Louise K. Hosking, Sharon A. Shellard, Bridget T. Hill
      Pages 253-260
    8. Maureen E. Harper
      Pages 261-269
    9. M. J. O’Hare
      Pages 271-286
  4. Back Matter
    Pages 287-299

About this book

Introduction

Growing human cancer cells in primary culture requires patience, intuition, care and experience. This is one of the few areas where the wrinkled senior scientist can be more productive than the bright young post-doc. There are few mechanical aids, no automated procedures, and kits are unheard-of. There is no right way to do it and every tumour is different. But this book will make it easier! Chapter 1 on characterization is essential reading. Much published work is useless because of the failure to take two simple steps to characterise the cells. The first step is to fully record all the clinical data - the absence of this information can render the work valueless. The second step is to confirm the origin of the tissue to exclude cross­ contamination. The wastage of years of work can be avoided with the use of a simple DNA preparation with a couple of commercially-available probes. Chapter 2 describes the development of serum-free media. This is a goal many would like to achieve, particularly if someone else does it, as it is laborious and empirical. Defined serum-free medium is essential for studies of growth factors and has major advantages in the commercial preparation of cell products and other applications.

Keywords

cancer

Editors and affiliations

  • J. R. W. Masters
    • 1
  1. 1.Institute of UrologyUniversity CollegeLondonUK

Bibliographic information